Browsing by Author "Usman, R. Y."

Now showing 1 - 2 of 2
  • Item
    Activation of pro-apoptotic cells, reactive astrogliosis and hyperphosphorylation of tau protein in trimethyltin-induced hippocampal injury in rats
    (Association of Anatomical Societies of Africa, 2020) Okesina, A. A.; Ajao, M. S.; Buhari, M. O.; Afodun, A. M.; Okesina, K. B.; Usman, R. Y.; Sulaimon, F. A.,
    Neurodegenerative diseases cause neural cells to lose both the functional and sensory abilities as a result of genetic factors, proteopathies and mitochondrial dysfunction. Neurodegeneration forms the basis of most neurodegenerative disorders for example Alzheimer’s disease, Huntington’s diseases, and Parkinson’s diseases. The mechanism that underlines the process of neurodegeneration is not well understood. Understanding the process and mechanism involved in neurodegeneration might offer a better therapeutic approach to positively manage cases of neurodegenerative diseases. Therefore, this study’s target was to create an animal model to study neurodegeneration. Sixteen adult male Wistar rats were used in the study and divided into two groups. Control (0.2 mL of normal saline (NS)), and trimethyltin-treated (TMT, 8 mg/kg stat dose only). These animals underwent perfusion with 4% paraformaldehyde, brain excision and analysis of p53 antigen, GFAP and Bielshowsky on these tissues. The results showed that animals in the control group showed presence of activated p53 antigen, reactive astrogliosis, neurofibrillary tangles, and amyloid plaques within the cytoplasm of the hippocampal cells. Cornus Ammonis (CA2) and (CA3) showed more of the trimethylrtin injury than CA1 and CA4. This study thus revealed that, intra-peritoneal administration of single dose of 8mg/kg of trimethyltin can offer an attractive disease model to study some neurodegenerative diseases.
  • Item
    Phoenix dactylifera polyphenols ameliorates monosodium glutamate induced cell damage in the dentate gyrus
    (Krishna Institute of Medical Sciences, 2021) Usman, R. Y.; Sulaimon, F. A.; Okesina, A. A.; Imam, A.; Imam, A. L.; Ajao M. S
    Background: Monosodium Glutamate (MSG) use is quite alarming considering the cascades of toxicity that arises from it. However, this study demonstrated the possible ameliorative activities of polyphenols of Phoenix dactylifera (PPD) on MSG-induced dentate gyrus degeneration in male adult Wistar rats. Aim and Objectives: To check the effects of PPD on MSG induced dentate gyrus neuronal damage in adult male Wistar rats. Material and Methods: Groups A to D of adult male rats underwent 14-day treatment of Normal Saline (NS), 500 mg ̸ kg PPD, 4 mg ̸g of MSG only, and 4 mg̸g MSG and 500 mg/kg PPD (MSG+PPD) concurrently. Group E received 500 mg ̸ kg PPD for a 14-day period prior to another 14-day of 4mg ̸ gMSG (PPD then MSG). Results: PPD was able to ameliorate the toxic effect of MSG as evidence of better cellular integrity, minimal cell vacuolations, minimized dispersed Nissl bodies and deeply stained Nissl bodies were observed upon PPD administration. It was also observed that it reduced the proliferation of reactive oxygen species, proteins and DNAdamage in the cells of the dentate gyrus. Conclusion: The study concluded that PPD was able to ameliorate the degeneration induced by MSG in the dentate gyrus of Wistar rats