Browsing by Author "Raphael Terlumun Ikpe"

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    Prevalence of molecular markers associat with Plasmodium falciparum chloroquine and sulphadoxine/pyrimethamine in (three) malaria endemic local areas of Benue State, Nigeria
    (Department of Medical Microbiology and Parasitology,, 2024) Amase Nyamngee; Raphael Terlumun Ikpe; Mariam Kehinde Sulaiman
    Introduction: currently, malaria (primarily caused by 106 countries and is one of the most severe public health problems globally, leading the cause of deaths especially among children and pregnant women particularly in developing countries. This study determined the drug-resistance molecular markers in endemic local areas of Benue State, North-central Nigeria between June 2023 and September 2024. Methods: the conclusive diagnosis of P. falciparum stage of the parasite in Giemsa-stained blood smears examined under a compound microscope. The DNA (Deoxyribonucleic acid) extraction from Chelex extraction method. Nested polymerase chain reaction followed by Restriction Fragment Length Polymorphisms (PCR/RFLP) were used for the detection of resistance transporter (pfcrt), P. falciparum multidrug resistance 1 ( reductase (pfdhfr) and P. falciparum dihydropteroate synthase ( SPSS Version 24.00 and inferences were drawn for Statistical significance at P<0.05. Results: the results revealed well-characteri aminoquinolines and the antifolate drugs indicating a high prevalence of resistance: 41%, 60%, 51% and 47% of P. falciparum isolates at codons N86 Conclusion: the prevalence of resistance of isolates to antimalarial drugs was significantly high. Therefore, strategies to reduce multiple-strain infections should be implemented to improve antimalarial drug efficacy and reduce the rate of spread of drug resi Introduction Malaria is found in over 106 countries and is one of the most severe public health problems, affecting half of the world's population and being a leading cause of avoidable death, especially among children and pregnant w Malaria fever originated in Africa and spread throughout the continent as the nomadic lifestyle was abandoned for agriculture which accidentally created favourable habitats for 2 Amase Nyamngee, Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Science, College of Health Sciences, University of Ilorin, Ilorin, Nigeria currently, malaria (primarily caused by Plasmodium falciparum) remains prevalent in over 106 countries and is one of the most severe public health problems globally, leading the cause of deaths especially among children and pregnant women particularly in developing countries. This study e molecular markers in Plasmodium falciparum infection in three malaria central Nigeria between June 2023 and September 2024. P. falciparum was based on identifying the characteristic asexual stained blood smears examined under a compound microscope. The P. falciparum positive blood samples was done using tion method. Nested polymerase chain reaction followed by Restriction Fragment Length Polymorphisms (PCR/RFLP) were used for the detection of Plasmodium falciparum Chloroquine multidrug resistance 1 (pfmdr1), P. falciparum dihydrofolate dihydropteroate synthase (pfdhps). Data were analysed using SPSS Version 24.00 and inferences were drawn for Statistical significance at P<0.05. characterized molecular markers of P. falciparum resistance to the 4 aminoquinolines and the antifolate drugs indicating a high prevalence of resistance: 41%, 60%, 51% isolates at codons N86Y, K76T, S108N, N51I and A437G respectively. the prevalence of resistance of isolates to antimalarial drugs was significantly high. strain infections should be implemented to improve antimalarial drug efficacy and reduce the rate of spread of drug resistance.