Browsing by Author "Oluwasola, A."

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    A Comparative Study on the Antioxidant Potentials of Honey and Some Selected Beverages in Male Wistar Rats.
    (Faculty of Education, Al-Hikmah University, 2019) Oluwasola, A.; Ayinde, T.O.
    This study compared the antioxidant potentials of honey and some selected beverages in male wistar rats. Twenty five male wistar rats (220-240g) were assigned into 5 groups of 5 animals each, such that the rats in groups I, II, III, IV and V received orally 1mL distilled water, 0.2mg/kg body weight (BW) of honey, 0.2mg/kg BW of vitamin C, 0.3mg/kg body weight (BW) of zobo and 0.2mg/kg BW of cocoa power, respectively. Catalase (WBC), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using standard methods. There was significant (p<0.05) increase in catalase and superoxide dismutase (SOD) levels for all the treatment groups when compared with the control. The order of significant (p<0.05) increased in these two parameters among the treatment groups were: cocoa powder < vitamin c < zobo < honey respectively. However, there was significant (p<0.05) decrease in malondihydehyde (MDA) levels for all the treatment groups when compared with the control. The order of significant (p<0.05) decreased in MDA levels among the treatment groups were: honey < zobo < vitamin c < cocoa powder respectively. This study showed that honey, zobo, vitamin c and cocoa powder could stimulate the activities of antioxidant enzymes by preventing the formation of lipid peroxidation. The consumption of honey, zobo, vitamin c and cocoa powder are therefore recommended in order to potentiate the activities of antioxidant enzymes. In addition, honey has the highest antioxidant potential and could consume more than cocoa powder which has the lowest.
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    Melatonin Mitigates Oxidative stress in Ethanolic Extract of Cannabis-Treated Female Wistar Rats
    (Nigerian Society of Biochemistry and Molecular Biology, 2019) Oluwasola, A.; Ayinde, T.O.; Olayaki, L.A.
    Cannabis sativa (CS) is one of the commonly abused substances by women of childbearing age. About 3.2% females are cannabis smokers in Nigeria, with varied consequences of congenital abnormalities due to interplay between oxidants, oxidants and antioxidants. This study investigated the effects of melatonin and cannabis on oxidative stress in female rats. Twenty female rats were assigned into four groups of five animals each. Groups I, II, III and IV received orally 1ml distilled water, 2mg/kg body weight (bw) of ethanolic extract of C. sativa (EECS), 2mg/kg bw of EECS plus 4mg/kg bw of melatonin and 4mg/kg bw of melatonin, respectively. Serum level of Superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase, total antioxidant capacity (TAC), lactate dehydrogenase and Malondialdehyde (MDA) were assayed using standard methods. CS significantly (p<0.05) decreased catalase, SOD, glutathione peroxidase, glutathione reductase and TAC levels. But it significantly (p<0.05) increased lactate dehydrogenase and MDA levels. All these effects were ameliorated to the level comparable to the control when the extract was administered in combination with melatonin. CS showed alterations of biochemical parameters which was probably mediated by oxidative stress. However, these effects were ameliorated by melatonin. Since the consumption of CS is increasing globally because of its medical uses, thus, consumption of melatonin as supplement may be recommended.
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    Nauclea latifolia (African peach) attenuates gastric acid secretion in rats with ethanol-induced ulceration
    (Published by Faculty of Health Sciences, Al-Hikmah University, Ilorin, Nigeria, 2021) Balogun, M. E.; Umahi, G. O.; Ayinde, T. O.; Oluwasola, A.; Busari, A. O.; Ajibola, K. A.; Yusuf, R. O.; Ojulari, L. S.
    Objective: The ethanolic leaf extract of N. latifolia (EENL) was investigated for its gastric acid antisecretory effects on ethanol-induced gastric ulcerated rats using an in vivo assay. Methods: Gastric mucosal ulceration was induced experimentally in Groups 2 to 5 rats using ethanol. Five groups of rats were orally pretreated with 3% Tween-80 only as vehicle group, 3% Tween-80 as ulcer group, 20 mg/kg of omeprazole as positive group, 250 and 500 mg/kg of EENL as the test groups, respectively before ulcer induction. Gastric acid secretion parameters and ulcerated areas were evaluated. Results: Pretreatment with EENL showed significant (P < 0.05) reduction in gastric volume, total acidity and ulcer index while the pH of gastric content increased significantly (P < 0.05) in rats with ethanol-induced ulcer. Ulcer group exhibited significantly severe mucosal injury and EENL or omeprazole offered significant (P < 0.05) protection against gastric mucosal injuries. Conclusion: The study suggests that EENL attenuates gastric acid secretion and ulceration induced by ethanol in Wistar rat. The observed pharmacological activities might be mediated via antisecretory and cytoprotective mechanisms.