Browsing by Author "Olumoh-Abdul, H.A"
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Item ACUTE AND SUBACUTE TOXICITY STUDIES ON METHANOL LEAF EXTRACT OF TURRAEA VOGELII HOOK. F. EX. BENTH.(Nigerian Journal of Pharmaceutical Sciences, 2018-03) Ayanniyi, R.O; Olumoh-Abdul, H.A; Ojuade, F.I; Adebisi-Jose, G.O; Rasheed, M.WIn Africa, majority of people rely on medicinal plants for their health care needs as well as for food. There are numerous scientific reports on efficacy of medicinal plants, with limited reports on the safety of these plants. Turraea vogelii Hook. f. ex. Benth. is an ethno medicinal plant indigenous to Tropical Africa. It is used as food, drinks and widely explored in ethno medicine for treatment of various ailments including; wounds, stomach ache, malaria fever, intestinal worms and urogenital infections. The aim of this study was to evaluate the acute and subacute oral toxicity of methanol leaf extract of T. vogelii in experimental animals. Acute oral toxicity was determined following OECD guideline 423. Doses of 125, 250, 500 mg/kg were administered for 28 days in the subacute oral toxicity study. Effect of extract on haematological parameters, liver and kidney function markers were determined. The acute oral toxicity of Turraea vogelii was estimated to be greater than 2000 mg/kg. The extract had no significant effect on haematological parameters, liver and renal function parameters when compared with the control. The extract however produced inflammatory changes and alterations in morphology of the liver. The results from this study shows that the methanol leaf extract of Turraea vogelii produced toxic effects on the liver when repeated oral doses were administered.Item Analgesic and Anti-Inflammatory Properties of the Methanol Stem Bark Extract of Lannea acida (A.Rich) in Laboratory Animals.(Faculty of Pharmaceutical Sciences, University of Ilorin, Ilorin., 2022) Aiyelero, O.M.; Olumoh-Abdul, H.A; Ojuade, F.I.; Yaro, A.H.; Muhammad, Z.Lannea acida is a medicinal plant used for the treatment of a wide range of ailments including haemorrhoid. In the present study, the phytochemical constituents present in the methanol stem bark extract of Lannea acida were determined using standard protocol. The analgesic and anti-inflammatory activities of the extract were evaluated. Acetic acid-induced writhing and hot plate-induced tests in mice were used to evaluate the analgesic activity, while Egg albumin-induced paw edema in rats was used to evaluate the anti-inflammatory activity. Median lethal dose of the extract was estimated using Lorke’s method. For each of the tests, thirty animals were divided into five groups of six mice each. Groups I – V received normal saline (10 ml/kg), Lannea acida extract (20, 40, and 80 mg/kg i.p.), and piroxicam (20 mg/kg) for acetic acid induced writhes/egg albumin-induced paw edema and pentazocine (20 mg/kg) for hot plate induced pain tests respectively. The phytochemical screening revealed the presence of carbohydrates, flavonoids, glycosides, alkaloids, saponins and tannins. The intraperitoneal LD50 value in mice was estimated to be 282.8mg/kg. The extract significantly (P<0.05) inhibited acetic acid-induced writhing and also produced more than 70% inhibition at all tested doses after 1 hour against thermal-induced pain in mice. The extract at all tested doses afforded 64% inhibition of paw edema at the end of the 4th hour. The result obtained lends the credence to the ethnomedical use of the plant in the management of haemorrhoids.Item Evaluation of anti-nociceptive and anti-inflammatory activities of leaf extract of Turraea vogelli Hook. f. ex. Benth(Pakistan Journal of Pharmaceutical Scences, 2019-01) Ayanniyi, R.O; Ojuade, F.I; Olumoh-Abdul, H.A; Salawu, M.K; Olufadi-Ahmed, Haishat; Adebisi-Jose, O. GbemisolaThe leaf extract of Turraea vogelii Hook. f. ex. Benth. is used in ethnomedicine for the management of pain and inflammation. Anti-nociceptive activity was determined using acetic acid-induced mouse writhing model. The antiinflammatory activity was investigated using in-vitro bovine serum albumin (BSA) denaturation assay and BSA-induced hind paw edema in rats. The extract (125-500 mg/kg) administered via the oral route produced a significant (p<0.005) inhibition of acetic acid-induced writhes. The percent inhibition of writhes for extract (500 mg/kg) and diclofenac (10 mg/kg) was 53.3 and 59.5% respectively. The methanol extract (10-6 -1.0 µg/mL) inhibited protein denaturation with IC50values of (1.06 × 10-3 µg/mL and 2.58 × 10-3 µg/mL) for extract and diclofenac respectively. Furthermore, the leaf extract (62.5 mg/kg) significantly (p<0.05) inhibited BSA-induced paw edema in rats. The methanol leaf extract of T. vogelii has anti-nociceptive and anti-inflammatory activities. These findings justify the use of the plant in traditional medicine for the management of pain and inflammation.Item Evaluation of Nutraceutical content and topical Anti-inflammatory Activities of Turraea vogelii Hook F. Ex Benth (Meliaceae)(Iranian Journal of Toxicology, 2019-02) Olumoh-Abdul, H.A; Ayanniyi, R.O; Ojuade, F.I; Agbana, R.JBackground: Turraea vogelii has been used as traditional medicine for the treatment of ailments including wounds, stomach aches, malaria, infections, filariasis cutaneous, among others and, it serves as source of food. The present study aimed to evaluate the nutraceuticals content and topical anti-inflammatory effect of hydro-methanol twig extract of Turraea vogelii (METV). Method: METV was obtained using cold maceration with water and methanol in ratio 30:70. The concentration of vitamins A, C and E, total phenolic and total flavonoid contents were evaluated by UV spectrophotometric method. Phenol and xylene-induced ear edema was used to evaluate the topical anti-inflammatory effect in mice. Results: The concentration of vitamins A, C and E was found to be 0.113 0.006, 9.303 0.012 and 0.020 0.001 mg in 100g of dried plant materials, respectively. Total phenolic and flavonoid contents were 0.281 0.318 mg/g gallic acid equivalent and 0.112 mg/g quercetin equivalent, respectively. Topical application of METV at a dose of 25 and 50mg/ear in xylene-induced ear edema produced significant inhibition (P<0.05) of 33 and 54% while in phenol-induced ear edema was significantly (P< 0.05) reduced by 44% and 34%. Dexamethasone (0.1mg/ear) produced an inhibition of 83% and 57% in phenol-induced ear edema. Conclusion: Results from this study demonstrated that Turraea vogelii twigs contain flavonoids and phenolic compounds, which may be responsible for the topical anti-inflammatory effect of the plant extract.Item Oral Ingestion of Cannabis sativa: Risks, Benefits, and Effects on Malaria-Infected Hosts(Cannabis and Cannabinoid Research, 2018-11-22) Akinola, Olugbenga; Ogbeche, O.Elizabeth; Olumoh-Abdul, H.A; Alli-Oluwafuyi, O. Abdulmusawwir; Oyewole, L. Aboyeji; Amin, Abdulbasit; AbdulMajeed, I. Wahab; Olajide, J. Olayemi; Nafiu, B. Abdurrazaq; Njan, A. Anoka; Olorundare, E. Olufunke; Gbotosho, O. GraceBackground: The emergence of a multidrug-resistant strain of Plasmodium falciparum (Pf Pailin) raises concern about malaria control strategies. Unfortunately, the role(s) of natural plants/remedies in curtailing malaria catastrophe remains uncertain. The claims of potential antimalarial activity of Cannabis sativa in vivo have not been well established nor the consequences defined. This study was, therefore, designed to evaluate the effects of whole cannabis consumption on malaria-infected host. Methods: Thirty mice were inoculated with dose of 1 · 107 chloroquine-resistant Plasmodium berghei ANKAinfected erythrocyte and divided into six treatment groups. Cannabis diet formulations were prepared based on weighted percentages of dried cannabis and standard mice diet and the study animals were fed ad libitum. Chemosuppression of parasitemia, survival rates, parasite clearance, and recrudescence time were evaluated. Histopathological studies were performed on the prefrontal cortex (PFC) and hippocampus of the animals after 14 days’ consumption of cannabis diet formulation by naive mice. Results: There was a significant difference ( p < 0.05) in the day-4 chemosuppression of parasitemia between the animals that were fed C. sativa and chloroquine relative to the untreated controls. There was also a significant difference in the survival rate ( p < 0.05) of animals fed C. sativa diet (40%, 20%, 10%, and 1%) in contrast to control animals on standard mice diet. A parasite clearance time of 2.18 – 0.4 was recorded in the chloroquine treatment group, whereas recrudescence in chloroquine group occurred on day 7. There were slight histomorphological changes in the PFC and cell densities of the dentate gyrus of the hippocampus of animals that were fed C. sativa. Conclusions: C. sativa displayed mild antimalarial activity in vivo. There was evident reduction in symptomatic manifestation of malaria disease, though unrelated to levels of parasitemia. This disease tolerance status may be beneficial, but may also constitute a transmission burden through asymptomatic carriage of parasites by habitual cannabis users.Item The Protective Effect of Croton zambesicus against Carbon Tetrachloride-induced Renal Toxicity in Rats(Iranian Journal of Toxicology, 2019-02) Ayanniyi, R.O; Olumoh-Abdul, H.A; Ojuade, F.I; Abdullahi, R.ABackground: The leaf extract of Croton zambesicus (CZ) is used in traditional medicine for the management of various conditions including kidney disease. The purpose of this study was to determine the protective effects of leaf extract of CZ on rat kidney toxicity induced by carbon tetrachloride. Method: Male albino rats were divided into 6 groups of 5 rats and treated for 5 days with aqueous extract (200-400 mg/kg) and n-butanol fraction (20-40 mg/kg) of CZ. Carbon tetrachloride (2 ml/kg of 40% in olive oil) was administered subcutaneously for 3 days and the rats were treated with the extract and fraction for 3 days. Animals were euthanized on day 8 with intraperitoneal injection of ketamine (60 mg/kg) and xylazine (7.5 mg/kg). Blood sample was collected for the analysis of serum electrolytes, urea and creatinine. Kidney tissue was harvested to determine the antioxidant enzyme activity. Results: Carbon tetrachloride produced increased serum sodium ion, chloride ion, urea and creatinine with decreased superoxide, catalase, glutathione, oxidized glutathione and an increase in malondialdehyde concentration. Treatment of rats with the aqueous leaf extract and n-butanol fraction attenuated the toxic effects of carbon tetrachloride on kidney with a significant decline in serum electrolytes, urea, creatinine and a significant increase in the concentration of antioxidant enzymes. Conclusion: Results from this study revealed that the aqueous leaf extract and n-butanol fraction of CZ had protective effect against carbon tetrachloride-induced renal toxicity in rats, thus justifying the consideration of this plant for the management of kidney disease.