Browsing by Author "Olayaki L.A.,"

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    Effect of testosterone supplementation on renal function in male albino rats.
    (Faculty of Basic Medical Sciences, Lagos State University College of Medicine, 2018) Ayinde, T.O.,; Olayaki L.A.,; Ojulari, L.S.,; Afodun, A.M.,; Quadri, K.K.; Fapounda, T
    Objectives: Testosterone is an anabolic steroid and it is secreted primarily by the testicles in males. Androgens have been shown to increase tubular sodium and water reabsorption and activate various vasoconstrictor systems in the kidneys. Testosterone increases blood pressure and may also influence renal electrolyte excretion. Methods: Fifteen adult male rats weighing 100 – 150g were randomly divided into three groups of five (5) rats each. Group 1 rats (Control) were given normal saline intramuscularly (IM). Group 2 rats were given 3mg/kg body weight (bw) of testosterone IM while Group 3 rats were given 9mg/kg bw testosterone IM for two weeks. All rats were allowed normal chow and water ad libitum. The animals were sacrificed under anaesthesia and blood samples collected through cardiac puncture. Results: Plasma urea levels of both low (2.02±0.23mmol/L) and high (10.3±0.61mmol/L) dose testosterone-treated animals were higher when compared with the Control group (1.94±0.07mmol/L), but the difference was only significant (p<0.05) in the high dose group. Asignificant (p<0.05) increase in plasma creatinine level in animals injected with low dose testosterone (33.0±2.46µmol/L) and high dose testosterone (46.0±5.67µmol/L) was observed when compared to the control group (25.2±3.91µmol/L). Conclusion: It was concluded from this study that exogenous administration of testosterone may alter normal kidney functions shown by elevated levels of urea and creatinine, but these could also be dose depend
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    Hepatoprotective effect of tryptophan in Carbontetrachloride-induced hepatotoxicity in male wistar rats
    (Society of Basic and Applied Physiology, Ahmedabad, India. Available, 2021) Ayinde T.O,; Olayaki L.A.,; Ojulari, L.S.,; Oluwasola A.,; Abdulraheem H.A.; Lawal, A.Z. &; Alli-Oluwafuyi, A.
    In the present study, tryptophan was evaluated for its hepatoprotective effects against carbontetrachloride-induced hepatocellular injury in rats. Hepatotoxicity was induced in male Sprague-Dawley rats by intraperitoneal injection of CCl4 (4ml/kg) in olive oil (1:1). Tryptophan at doses of 100mg/kg and 200mg/kg was administered orally for 28 days. The hepatoprotective effect of tryptophan was evaluated by the assay of biochemical parameters viz.: alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), total protein, albumin and lipid peroxidation. Tryptophan produced a dose-dependent significant increase (p<0.001) in serum ALP (41% & 60%), a dose-dependent decrease (p<0.001) in serum Malondialdehyde (61% & 65%), and a significant increase (p<0.001) in levels of serum protein and serum albumin, in CCl4induced hepatotoxic rats, following administration of 100 mg/kg bwand 200 mg/kg bw, respectively. The toxic effect of CCl4 in tryptophan treated groups was controlled significantly by restoration of the levels of enzymes, total protein and albumin as compared to the CCl4 treated groups. The results suggest that tryptophan is able to significantly alleviate the hepatotoxicity induced by CCl4 and may be attributed to the antioxidant property of tryptophan.