Browsing by Author "Oladoye, C.O."

Now showing 1 - 2 of 2
  • Item
    Citric Acid Production by Aspergillus niger and Trichoderma longibrachiatum using Ananas comosus Waste and Saccharum officunarum Bagasse
    (Nigerian Society of Biochemistry and Molecular Biolog, 2014-01-01) Omojasola, P.F; Okun, H.A.; Oladoye, C.O.; Kayode, R.M.O.; Ahmed El-Imam, Amina
    Trichoderma longibrachiatum and Aspergillus niger were used to ferment two agro-based wastes; Pineapple peel (PP) and sugarcane bagasse (SB) as substrates for citric acid production. The wastes were ground, used to as carbon source in mineral salts media. Submerged fermentation was conducted at (29±10C) for five days and citric acid production and residual glucose were evaluated. pH, substrate concentration and inocula size were varied to determine their effect on citric acid production. 0.62 and 0.61 g L-1 of citric acid were produced from PP; while 0.08 and 0.24 g L-1 were produced from SB by T. longibrachiatum and A. niger respectively on Day 5 of fermentation. Maximum citric acid yield by A. niger was 0.61 g L-1 at pH 3; 0.92 g L-1 at 25% substrate concentration and 0.70 g L-1 at 0.5% inocula size on PP and 0.24 g L-1, 0.5 g L-1 and 0.52 g L-1 respectively on SB. Maximum yield with T. longibrachiatum was 0.6 g L-1 at pH 3; 0.62 g L-1 at 15% substrate concentration and 1.26 mg mL-1 at 0.5% inocula size on PP and 0.24 g L-1, 0.50 g L-1 and 0.52 g L-1 at pH 3.0, 25% substrate concentration and 1.5% inocula size respectively on SB. Under optimized conditions, T. longibrachiatum yielded 0.46 g L-1 citric acid which was significantly higher (P<0.05) than 0.41mg mL-1 produced by A. niger on PP. No significant difference (P<0.05) in the yield by both organisms on SB. Both wastes were found to be suitable substrates for commercial citric acid production.
  • Item
    βeta-lactamase production and plasmid profiling of penicillins resistant Staphylococcus aureus isolated from food handlers.
    (International Moringa and Nutriceutical Research Society, University of Ilorin, Ilorin, Nigeria, 2015) Kayode, R.M.O; Adedeji, B.S.; Oladoye, C.O.; Laba, S.A.; Salami, K.O
    This study investigated the prevalence and plasmid profiles of βeta-lactamase positive Staphylococcus aureus isolated from the nasal and hand swabs of some healthy food handlers in Tanke area of Ilorin, Nigeria. Sensitivity test of the isolated strains of Staphylococcus aureus was carried out with six different types of penicillin based antibiotics using standard procedures. The plasmid profile was carried out using Agarose gel electrophoresis and the result shown in Polaroid gel instant camera through an orange filter. The photograph was develop and observed for bands. There was 100 % occurrence of S. aureus from both nasal and hand swab samples. The result reveals 31.57% βeta-lactamase positive Staphylococcus aureus (βPSA) from the nasal swabs, while it was 15.78% from the hand swabs. The strains of Staphylococcus aureus isolated from the nose and hand swabs were resistant to most of the selected penicillin based antibiotics in the following decreasing order: Penicillin (100 %), Ampicillin (86.84 %), Amoxicillin (73.68 %), Ampicloxacillin (68.42 %), Gentamycin (34.02%) and Erythromycin (15.78%). The results of plasmid profiles of the βeta-lactamase positive Staphylococcus aureus (βPSA) strains showed that 15.7% had distinct plasmid bands. Although, less than half of the total isolated Staphylococcus aureus strains were βPSA (23.6%) and 66.6% of the total βPSA carried distinct plasmid bands; which is an indication of a potential microbiological hazards in foods prepared by the food handlers especially, if proper hygienic condition is not maintained during food preparations.