βeta-lactamase production and plasmid profiling of penicillins resistant Staphylococcus aureus isolated from food handlers.
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Date
2015
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Journal ISSN
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Publisher
International Moringa and Nutriceutical Research Society, University of Ilorin, Ilorin, Nigeria
Abstract
This study investigated the prevalence and plasmid profiles of βeta-lactamase
positive Staphylococcus aureus isolated from the nasal and hand
swabs of some healthy food handlers in Tanke area of Ilorin, Nigeria. Sensitivity
test of the isolated strains of Staphylococcus aureus was carried out
with six different types of penicillin based antibiotics using standard procedures.
The plasmid profile was carried out using Agarose gel electrophoresis
and the result shown in Polaroid gel instant camera through an orange
filter. The photograph was develop and observed for bands. There was 100
% occurrence of S. aureus from both nasal and hand swab samples. The
result reveals 31.57% βeta-lactamase positive Staphylococcus aureus
(βPSA) from the nasal swabs, while it was 15.78% from the hand swabs.
The strains of Staphylococcus aureus isolated from the nose and hand
swabs were resistant to most of the selected penicillin based antibiotics in
the following decreasing order: Penicillin (100 %), Ampicillin (86.84 %),
Amoxicillin (73.68 %), Ampicloxacillin (68.42 %), Gentamycin (34.02%)
and Erythromycin (15.78%).
The results of plasmid profiles of the βeta-lactamase positive Staphylococcus
aureus (βPSA) strains showed that 15.7% had distinct plasmid bands.
Although, less than half of the total isolated Staphylococcus aureus strains
were βPSA (23.6%) and 66.6% of the total βPSA carried distinct plasmid
bands; which is an indication of a potential microbiological hazards in
foods prepared by the food handlers especially, if proper hygienic condition
is not maintained during food preparations.
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Keywords
βeta-lactamase, penicillins, plasmid profile, food handlers, Staphylococcus aureus