Browsing by Author "Odebisi-Omokanye, Mutiat Busayo"

Now showing 1 - 8 of 8
  • Item
    Analysis of Indoor Air Microflora of Some Daycare Centres in Ilorin South Local Government Area, Nigeria
    (Al-Hikmah Journal of Pure and Applied Sciences, 2017) Sule, Ismaila Olawale; Agbabiaka, Toyin Olayemi; Saliu, Kudirat Bolanle; Odebisi-Omokanye, Mutiat Busayo; Zakariyah, Rahmat Folashade; Ali, Aminat A
    The microflora of air in ten daycare centres within Ilorin South Local Government Area of Kwara State, Nigeria was investigated by settling plate technique. The plates were exposed for a period of 10 minutes at each sampling point. The counts of bacteria, total coliform and fungi ranged from 10 to 200, 10 to 170 and 6 to 110 cfu/10 minutes respectively. A total of 23 microorganisms comprising 12 bacteria and 11 fungi were isolated in this study. These bacteria were Micrococcus sp., Staphylococcus arlettae, Corynebacterium pseudotuberculosis, Staphylococcus haemolyticus, Corynebacterium kutscheri, Staphylococcus warneri, Staphylococcus aureus, Corynebacterium diphtheriae., Corynebacterium amycolatum, Bacillus pumilus, Corynebacterium matruchotii and Listeria grayi while the fungi were Aspergillus fumigatus, Aspergillus niger, Penicillium chrysogenum, Penicillium sp., Aspergillus flavus, Rhizopus stolonifer, Fusarium oxysporum, Saccharomyces cerevisiae, Alternaria alternata, Aspergillus glaucus and Polyporus sp. The predominant bacterium and fungus in this study were Micrococcus sp. and Aspergillus fumigatus respectively. It can be concluded that the indoor air of the daycare centres used for this study had diverse species of Gram positive bacteria, high occurrence of non-pathogenic bacteria and fungi. Moulds dominate the fungal isolates and some of the bacterial species are commensals found on human body whereas the fungi are environmental in origin. A few pathogenic species were isolated among the bacteria and fungi.
  • Item
    The antibacterial activity of the leaf and stem bark extract of Eucalyptus citriodora (Hook)
    (Federal University Kashere, 2014) Odebisi-Omokanye, Mutiat Busayo; Adeyemi, Sherif Babatunde; Komolafe, Shakirat Oyindamola; Olahan, Ganiyu Shittu; Gbadamosi, Idayat Titilayo
  • Item
    Antibacterial Evaluation of Extracts of Seed Kernel and Leaf of Moringa Oleifera Against Bacteria from Raw Cow Milk
    (Nigerian Journal of Microbiology, 2016) Zakariyah, Rahmat Folashade; Sani, Al-hassan; Odebisi-Omokanye, Mutiat Busayo; Ahmed, Risikat Nike
    Moringa oleiferahas been found very useful in a lot of health related problems owing to its medicinal components and values, these has led to research on its antibacterial activity against food borne pathogens. Aqueous and acetone extracts of Moringa oleifera seed kernel and leaf were evaluated for antibacterial activity against Bacillus cereus, Staphylococcus aureus and Escherichia coli isolated from raw cow milk. The antibacterial assay was carried out at concentrations of 250, 125, 62.5 and 31.25mg/ml using modified agar well diffusion method. Aqueous extract inhibited the growth of test isolates at varying degrees. Aqueous extract of seed kernel was highly inhibitory on Staphylococcus aureus with a zone of inhibition (23.33±1.20mm) at concentration of 250mg/ml. Minimum Inhibitory Concentration showed that both extracts inhibited the growth of Bacillus cereus, Staphylococcus aureus and Escherichia coli at concentrations ranging from 125 to 21.25mg/ml. The antibacterial effect of standard antibiotics was performed and it was observed that Gentamycin inhibited growth of the three test isolates. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, steroids, saponin, tannin, phenols, glycosides and carbohydrates compound in the extracts. From the present study, Moringa oleifera seed kernel and leaf can be a promising source of phytochemical components and further studies is therefore recommended
  • Item
    Evaluation of the Antibacterial Efficacies of Hand wash Disinfectants on Pathogenic Bacterial Hand Swab Isolates of Health workers in Ilorin, Nigeria
    (Department of Biological Sciences Federal University of Technology, Minna., 2016) Odebisi-Omokanye, Mutiat Busayo; Sule, Ismaila O; Zakariyah, Rahmat Folashade; Jimoh, Faosat A; Olugbade, O.F
    Hands are the most implicated in the transmission and spread of pathogens that causes disease. Hand hygiene has been said to be the most important way to avoid these infections. Hand washing with the use of hand wash is one of the ways to tackle the barriers to efficient hand hygiene. This study assessed the efficacy of seven popular brands of Hand washes (HW): DET, SAV, CHE, VIS, NIV, DOV and CAR (all abreviations in full for the first time) against some clinically important bacterial pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis recovered from hands of health care workers. The antibacterial susceptibility and minimum inhibitory concentration of the hand washes was determined using the agar diffusion and broth dilution method, respectively. Each brand showed different activities against the isolates. VIS had the highest inhibitory effect against all organisms it was tested on, while SAV was the least effective. S. aureus was the most susceptible test organism, with the highest susceptibility to CAR (32.5 mm). P. mirabilis was most resistant, with the highest resistance to DET (9.0 mm). Antibacterial activity of the hand wash decreased with increased dilution. The minimum bactericidal concentration (MBC) was obtained for all the hand washes when not diluted. It is, therefore, recommended that the dilution of hand wash which is a commonly done in most households, hospitals, offices and eateries should be discontinued as these products are not active when diluted.
  • Item
    MICROBIOLOGICAL AND PHYSIOCHEMICAL ASSESSMENT OF STREET VENDED FRIED SOYABEAN CHEESE SOLD WITHIN ILORIN METROPOLIS, NIGERIA.
    (Journal of Science, Technology, Mathematics and Education (JOSTMED), 2016-03) Odebisi-Omokanye, Mutiat Busayo; Zakariyah, Rahmat Folashade; Jimoh, Fausat Abimbola; Olugbade, O.F
    The microbiological and physicochemical quality of street vended fried soyabean cheese products from five different locations was assessed. Standard pour plate technique was used to examine the microbial load of the soyabean cheese. The pH ranged from 6.48 + 0.241 – 6.58 + 0.192, Moisture content ranged from 13.94% + 0.152 – 14.22% + 0.179. The viable bacterial counts ranged from 7.0 to 61 x 105 cfu/g, while the fungal load ranged from 1 to 40 x 103 cfu/g. Eight bacteria species were isolated and identified as Bacillus subtilis, Escherichia coli, Micrococcus sp., Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter aerogenes and Klebsiella pneumoniae. Similarly, four fungal species were isolated and identified as Mucor racemosus, Aspergillus fumigatus, Saccharomyces cerevisiae, and Rhizopus stolonifer. The results suggest that the soyabean cheese samples were contaminated with pathogenic microorganisms and could cause health hazard to the consumers. There is the need to improve personal hygiene and environmental sanitation during and after production of the soyabean cheese.
  • Item
    PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL EFFECT OF SEEDS OF Monodora myristica ON SELECTED MICROORGANISMS
    (2020) Jimoh, Rahmat Folashade; Ajao, A.T; Zakariyah, Rahmat Folashade; Odebisi-Omokanye, Mutiat Busayo; Abdulrahman, Halimat O
    The increase in the prevalence of effects of many synthetic antimicrobial agents and incidence of multiple drug resistant microorganisms has spurred scientists on the research for plant-based antimicrobial of therapeutic potentials. The Monodora myristica has been used in Nigeria traditionally as condiments and has been harnessed as a therapeutic agent in the treatment of skin infection and dysentery. Aqueous, n-hexane and ethanolic extracts of the seeds was screened for antimicrobial activity against pathogenic microorganisms implicated in causing vagina infections. This finding showed that the efficacy of the extracts was concentration dependent. Aqueous, ethanolic and N-hexane extract showed varying degree of inhibition with different magnitude against the test isolates. Ethanolic extract showed highest activities against Candida albican, followed by Staphylococcus aureus and Klebsiella pneumoniae with 21.5mm, 19.5 mm each respectively. The same patterns of of inhibition was also observed in N-hexane extract. The antimicrobial effect of the two extracts were statistically not significant. The extracts had minimum concentration that ranged between 150mg/ml and 200mg/ml. The ethanolic extract showed more inhibitory effects compare to the aqueous and n- hexane extracts. The ethanolic extract found to contain highest quantity of phytochemicals while aqueous extract has lowest amount of the phytochemicals. It is therefore can be affirmed that Monodora myristica seed extracts could be exploited as therapeutic agents to drug resistant microorganisms.
  • Item
    SAFETY EVALUATION OF SOME READY-TO-USE HERBAL CONCOCTIONS SOLD WITHIN ILORIN METROPOLIS
    (Nassarawa State University, Keffi, 2020-03) Odebisi-Omokanye, Mutiat Busayo; Saliu, Bolanle Kudirat; Abdulrauf, Lukman Bola; Jimoh, Faosat Abimbola; Adebiyi, Opeyemi Adeola; Zakariyah, Rahmat Folashade
    Safety and quality of indigenous herbal concoctions in Nigeria is of public health concern as the consumption is on the increase. Hence, this study was conducted to evaluate microbial and heavy metals contaminants in selected ready –to- use herbal concoctions within Ilorin metropolis. The heterotrophic microbial counts using pour plate method were assessed and the contaminations of selected heavy metals were analyzed using Atomic Absorption spectrophotometer. The viable plate count for bacteria and fungi ranged between 2.0 ×104 - 10.5 ×105 CFU/ml and 2.0 ×104- 8.3×105 respectively. Bacteria and fungi isolated include Pseudomonas aeruginosa, Micrococcus luteus, Citrobacter freundii, Staphylococcus aureus, Klebsiellia pneumoniae, Corynebacterium kutscheri, Aeromonas hydrophilia, Staphylococcus epidermis, S. cerevisae, Gliocladium sp., Penicillum sp., A. alternata, Aspergillus niger, C. albicans, R. stolonifer, Geotrichum sp and A. flavus respectively. Pb and Cu level concentrations were found to be in the range of 0.1-0.3mg/L and 0.01- 074 mg/L respectively. The concentration of Pb and Cu were generally high and above the safe limits set by WHO/FAO while Cd was not detected in any of the samples. Generally, the consumers of these herbal products are unwittingly exposed to heavy metal poisoning and microbial contaminants. It is suggested that regulatory bodies should intensify efforts to minimize human exposure risk.
  • Item
    SCREENING OF Prosopis africana FOR ANTIMICROBIAL ACTIVITY AGAINST SELECTED ORAL PATHOGENS
    (Nigeria Journal of Pure and Applied Sciences, 2017) Zakariyah, Rahmat Folashade; Sani, Al-hassan; Odebisi-Omokanye, Mutiat Busayo; Ahmed, Risikat Nike; Jimoh, Fausat Abimbola; Badmus, Simbiat Atinuke
    Prosopis africana, a chewing stick popularly used in the South Western part of Nigeria was examined for antimicrobial activity against Lactobacillus casei ATCC 334, Staphylococcus aureus ATCC 35218, Candida albicans ATCC 10231, Escherichia coli ST2747 and Streptococcus mutans using agar well diffusion method. Extraction was done using ethanol and distilled water. The plant was screened for its phytochemical components. Gas chromatography mass spectrometry analysis was done to detect the secondary metabolites. All the extracts showed antimicrobial activity against the organisms with zones of inhibition ranging from 3.33±0.29 - 13.83±1.61mm except Staphylococcus aureus ATCC 35218 which was resistant to aqueous extract of the dried sample. Statistical analysis of data showed that there was no significant difference in the antimicrobial effects of ethanolic and aqueous extracts of the plant samples at 95% confidence intervals. The phytochemical screening result showed that phenols, alkaloids, sterols, triterpenes and saponins are components of the chewing stick. Also, fatty acids such oleic, palmitic and stearic acids were chemical constituents detected in the plant extracts by GC-MS. The results obtained in this study indicate that P. africana will be useful in the treatment of oral infections.