Antibacterial Evaluation of Extracts of Seed Kernel and Leaf of Moringa Oleifera Against Bacteria from Raw Cow Milk

Abstract

Moringa oleiferahas been found very useful in a lot of health related problems owing to its medicinal components and values, these has led to research on its antibacterial activity against food borne pathogens. Aqueous and acetone extracts of Moringa oleifera seed kernel and leaf were evaluated for antibacterial activity against Bacillus cereus, Staphylococcus aureus and Escherichia coli isolated from raw cow milk. The antibacterial assay was carried out at concentrations of 250, 125, 62.5 and 31.25mg/ml using modified agar well diffusion method. Aqueous extract inhibited the growth of test isolates at varying degrees. Aqueous extract of seed kernel was highly inhibitory on Staphylococcus aureus with a zone of inhibition (23.33±1.20mm) at concentration of 250mg/ml. Minimum Inhibitory Concentration showed that both extracts inhibited the growth of Bacillus cereus, Staphylococcus aureus and Escherichia coli at concentrations ranging from 125 to 21.25mg/ml. The antibacterial effect of standard antibiotics was performed and it was observed that Gentamycin inhibited growth of the three test isolates. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, steroids, saponin, tannin, phenols, glycosides and carbohydrates compound in the extracts. From the present study, Moringa oleifera seed kernel and leaf can be a promising source of phytochemical components and further studies is therefore recommended

Description

Keywords

Food-borne pathogens, Minimum Inhibitory Concentration, Moringa oleifera, Phytochemical screening, Raw cow milk

Citation

Zakariyah et al., 2016

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