Browsing by Author "OLATUNJI, A.O."

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    Anti-anaemic effect of ethanol leaf extract of Cnidosculus aconitifolius on cyclophosphomide- induced anaemia in rats
    (DE GRUYTER, 2020-05-15) ATATA, J.A.; AYOOLA, T.O.; AJADI, A.A.; ADAMU, S.; OLATUNJI, A.O.; BIOBAKU, K.T.
    Background: The folkloric claim that Cnidoscolus aconiti- folius (Chaya) could ameliorate anaemia requires scientific revalidation in anaemic models. Objective: The aim of this study is to investigate the anti- anaemic effect of Cnidoscolus aconitifolius on cyclophos- phamide-induced anaemia in rats. Methods: The leaves of Cnidoscolus aconitifolius were collected, dried and extracted with ethanol. Twenty five Wistar Albino rats weighing 120–180 kg were used. Anaemia was induced in groups 2, 3, 4 and 5 with a single injection of cyclophosphamide, while group 1 served as a negative control without anemia and was treated with 0.5 mL of normal saline, Group 2 rats were treated with 100 mg/kg body weight of Cnidoscolus aconitifolius. Group 3 rats were treated with 500 mg/kg body weight of Cnido- scolus aconitifolius. Group 4 served as the positive control rats and was treated with 0.5 mL of normal saline. Group 5 which served as the standard control rats and were treated with 5 mg/kg body weight of standard drug Chemiron. The treated lasted for two weeks during which blood samples were collected from each rat for haematological analysis. Results: The result shows that hematological parame- ters were elevated in groups 2, 3 and 5 rats treated with 100 mg/kg and 500 mg/kg body weight of ethanol extract of Cnidoscolus aconitifolius and chemiron respectively. Conclusions: Ethanol leaf extract of Cnidoscolus aconiti- folius at a graded dose of 500 mg/kg body weight had higher ameliorative effect on the haematological parame- ters of cyclophosphamide-induced anemia in rats.
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    Effect of diet modification with iron-on haematological and biochemical parameters of non-anaemic puppies
    (College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Niger, 2024-12-14) ATATA, J.A.; OMOTOSHO, G.S.; AJADI, A.A.; ADAM, M.; AKANBI, O.B.; JEGEDE, H.O.; OLATUNJI, A.O.
    Dietary iron intake is vital for proper growth and development in puppies. The aim of this study was to examine the effects of diet modification with iron on haematological and biochemical parameters of puppies. A total of ten apparently healthy puppies weighing between 4.3 – 6.5 kg were used for this study. They were assigned to two groups of five dogs each. Group I was iron-supplemented, while group II served as non-iron supplemented control. Puppies in group I were fed diet modified with iron supplementation, while the group II puppies were fed with puppy food without supplementation. Result of this study revealed significant changes in haematological and biochemical parameters in the iron-supplemented compared to the control. Haemoglobin levels, erythrocyte counts, and the mean corpuscular volume showed significant increases (p0.05). Serum iron was elevated significantly (p0.05) in the iron-supplemented compared to the control group. However, the lipid profile, kidney function markers, oxidative stress biomarkers as well as the activities of the liver enzymes were comparable between the two groups (p>0.05). The findings of this research suggest that iron modified diets improved haematological parameters and iron status in puppies.