Browsing by Author "Momoh, E.O."
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Item ANALYSIS OF GENE TIC DIVERSITY IN MUSCOVY DUCK OF BARUTEN LOCAL GOVERNMENT KWARA STATE NIGERIA, USING THE NUCLEAR CYP2U1(Nigerian Journal of Genetics, 2021) Sola-Ojo, F.E.; Adeola, C.A.; Yusuff, O.A.; Momoh, E.O.The analysis of Genetic diversity within and between populations is important for development of sustainable livestock production. Muscovy duck (Carina Moschata) is important among the poultry specie raised by most peasant farmers in Baruten local government area of Kwara State, Nigeria for egg and meat production. This study evaluated the genetic diversity between Muscovy duck populations in some selected locations within the local government; [Bani (BON), Okuta (OKE), Yembeleku (BART) and Ilesha Baruba (ILE)] using the nuclear DNA Cytochrome P450 family 2 Subfamily U Polypeptide 1 (CYP2U1) genes. The results showed 722 monomorphic sites and 25 polymorphic sites. The sequences obtained were grouped into nine haplotypes with diversity of 0.771 and low nucleotide diversity per site (Pi-0.00608). A total number of 26 mutations were discovered with haplotype diversity ranging from 0.80 to 0.86 for ILE, BART and BON Muscovy duck. The Jalview results from 0 to 167 bp revealed some of the polymorphism consisting of substitutions and insertions in BON, BART and ILE at 33, 37, 47, 113 and 159 bp; while the phylogenetic tree showed a high level of monophyletic relationship between some of the Muscovy duck. Our study reveals high haplotype diversity but low nucleotide diversity within and between the Muscovy duck studied in Baruten local government of Kwara State, Nigeria. The high haplotype diversity is in accordance with the high number of haplotypes observed in each location, while the low nucleotide diversity reflects that Nigeria Muscovy duck are likely a descent of common maternal lineage. This finding serves as baseline information, thus more research evidence including wider geographic coverage of Nigeria is still required.Item Genetic diversity and relationship of Nigerian Muscovy duck populations(Korean Society of Animal Breeding and Genomics., 2021) Sola-Ojo, F.E.; Adeniyi, C.A.; Lameck, A.O.; Yusuff, O.A.; Momoh, E.O.; Adekoya, A.R.; Adedibu, I.I.Muscovy duck (Caraina Moschata) is an economically important species due its unique meat taste and low-caloric content. It is one of the domestic poultry species in Nigeria as it ensures food security to the rural sectors. Rearing of Muscovy is concentrated in some part of the country due to its peculiar nature as water loving poultry species and peoples belief. In this study, we analyzed the genetic diversity and relationship of Muscovy duck populations collected from 15 different locations where Muscovy duck lovers resides in four states (Kaduna, Kwara, Niger and Oyo) of Nigeria based on Nuclear DNA Cytochrome P450 Family 2 Subfamily U Member 1 (CYP2U1) gene. A total of 48 polymorphic sites consisting of substitutions with 33 singletons variables was observed. The 82 sequences were assigned into 32 distinct haplotypes. Haplotype diversity was 0.7925. The Phylogenetic analysis showed close clustering across all locations with the exception of BART 495, PAT 35 and KEY 764 individuals. The maternal genetic structure likely suggests the extensive genetic intermixing within the country. In addition, the differentiation of BART 495, PAT 35 and KEY 764 may be due to a certain demographic history and/or artificial selection that shaped its haplotype profile. The current data on Nigerian Muscovy duck genetic diversity based on nuclear DNA CYP2U1 gene do not permit us to make further conclusions; therefore, more research evidence from genetics and archaeology is still required.Item Molecular Sexing and Taxonomic classification of Nigerian Guinea Fowl using Chromo Helicase DNA Binding Gene and 12S mitochondrial rRNA gene.(The Korean Society of Animal Breeding and Genomics., 2021) Sola-Ojo, F.E.; Afolabi-Balogun, N.B.; Adeniyi, C.A.; Adeyemi, K.D.; Ayorinde, K.L.; Alli, O.I.; Oni, O.A.; Okeke, C.U.; Momoh, E.O.; Adewara, J.; Abdulkareem, I.Sexing of birds at early age is very important for efficient selection and breeding; while characterization and taxonomic identification is relevant in conservation of birds’ genetic resources. This study used the genomic DNA of ten (10) guinea fowl keets to determine their sex using agarose gel electrophoresis and sequencing with chromo helicase DNA (CHD) binding genes, they were also characterize taxonomically using 12S rRNA mitochondria genes. The results of this study shows a double band (ZW) for females and a single band (W) for males under Agarose gel electrophoresis view, the Guinea fowl keets sequenced showed some deletions and were closer to Gallus_CHD12 in the phylogenetic tree. The Taxonomic classification result shows that the sequenced guineafowl keets were most related to the Numida meleagris 12S mitochondrial ribosomal RNA. This study corroborate the fact sex of guineafowl keet can be easily identified at genomic DNA level and they can be characterized taxonomically using the 12SrRNA mitochondrial genes.