ANALYSIS OF GENE TIC DIVERSITY IN MUSCOVY DUCK OF BARUTEN LOCAL GOVERNMENT KWARA STATE NIGERIA, USING THE NUCLEAR CYP2U1
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Date
2021
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Nigerian Journal of Genetics
Abstract
The analysis of Genetic diversity within and between populations is important for development of sustainable livestock production. Muscovy duck (Carina Moschata) is important among the poultry specie raised by most peasant farmers in Baruten local government area of Kwara State, Nigeria for egg and meat production. This study evaluated the genetic diversity between Muscovy duck populations in some selected locations within the local government; [Bani (BON), Okuta (OKE), Yembeleku (BART) and Ilesha Baruba (ILE)] using the nuclear DNA Cytochrome P450 family 2 Subfamily U Polypeptide 1 (CYP2U1) genes. The results showed 722 monomorphic sites and 25 polymorphic sites. The sequences obtained were grouped into nine haplotypes with diversity of 0.771 and low nucleotide diversity per site (Pi-0.00608). A total number of 26 mutations were discovered with haplotype diversity ranging from 0.80 to 0.86 for ILE, BART and BON Muscovy duck. The Jalview results from 0 to 167 bp revealed some of the polymorphism consisting of substitutions and insertions in BON, BART and ILE at 33, 37, 47, 113 and 159 bp; while the phylogenetic tree showed a high level of monophyletic relationship between some of the Muscovy duck. Our study reveals high haplotype diversity but low nucleotide diversity within and between the Muscovy duck studied in Baruten local government of Kwara State, Nigeria. The high haplotype diversity is in accordance with the high number of haplotypes observed in each location, while the low nucleotide diversity reflects that Nigeria Muscovy duck are likely a descent of common maternal lineage. This finding serves as baseline information, thus more research evidence including wider geographic coverage of Nigeria is still required.
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Keywords
Diversity, Haplotype, Muscovy duck, Nucleotide, Polymorphism