Browsing by Author "Krishnamurthy, R."

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    Androgenic Effects of Aqueous seed extract of Moringa oleifera in Male Wistar Rats
    (Published by School of Medicine and Health Sciences, University of Papua, New Guinea, 2022) Ayinla M.T; Muhammad, A.S.; Ayinde, T.O.; Ojulari, L.S.; Owoyele, B.V.; Asuku, A.O.; Adebisi, R.O.; Badmus, O.A.; Krishnamurthy, R.
    Androgenic effects of Aqueous Seed-extract of Moringa oleifera (ASMO) in male Wistar rats were investigated. Eighteen (18) male Wistar rats weighing 200-240g were used for this study. The rats were divided into three (3) groups: Control (Group 1) that received 10 ml/kg of normal saline, Group 2: received low dose of ASMO (200mg/kg), and Group 3: received high dose of ASMO (500mg/kg). The animals were treated for twenty-eight days. On the 29th day, the rats were sacrificed and the testes were carefully removed for semen and biochemical analysis. Body weight, reproductive and vital organ weights were determined. Sperm parameters (motility, morphology, count and viability), tissue testosterone, luteinizing hormone (LH), Malondialdehyde (MDA) and Catalase were also determined using standard methods. Data were analysed using one-way ANOVA followed by Duncan new multiple range post hoc test. The result showed ASMO significantly increased (p<0.05) the final body weight, weight of reproductive and vital organs. Moreover, 200mg/kg body weight dose of ASMO significantly increased (p<0.05) the sperm parameters but 500mg/kg body weight dose significantly decreased it. ASMO also caused a significant dose dependent increase (p<0.05) in testosterone and catalase level but a significant decrease (p<0.05) in MDA and LH level compared with the control. In conclusion, this study revealed that ASMO has androgenic effects in male rats and thus provides a basis for the traditional use of Moringa oleifera in the management of male sexual disorders.
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    GENETIC DIVERSITY IN POPULATIONS OF DIPCADI FILAMENTOSUM MEDIK. USING ISSR MOLECULAR MARKERS
    (West University of Timisoara, Romania, 2018) Abdulkareem, K.A; Mustapha, O.T; Krishnamurthy, R.
    Genetic diversity was estimated among 13 populations of Dipcadi filamentosum Medik. Inter Simple Sequence Repeat (ISSR) was used to generate data to examine the patterns of genetic differentiation between and within the populations. A total of ten primers used generated 146 amplicon of which 97.9% of loci detected revealed polymorphism. The Shannon’s indices (I) and Nei’s genetic diversity (h) among the studied populations from the various regions were estimated at 0.6216 (SD = 0.1199) and estimate of gene flow (Nm) in the population was 5.0555. Cluster analysis (neighbor-joining, NJ) revealed that the North-West populations (KAT) are genetically distinct from the North-central populations (SOB, KAB, KAM and KAS). Ordination by Principal Component Analysis (PCA) supported the findings of NJ. The SOB and YOB; MAI and UNI populations clustered as closely related are probably as a result of correlation between the geographical locations and gene flow.