GENETIC DIVERSITY IN POPULATIONS OF DIPCADI FILAMENTOSUM MEDIK. USING ISSR MOLECULAR MARKERS
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Date
2018
Journal Title
Journal ISSN
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Publisher
West University of Timisoara, Romania
Abstract
Genetic diversity was estimated among 13 populations of Dipcadi filamentosum Medik. Inter Simple Sequence Repeat (ISSR) was used to generate data to examine the patterns of genetic differentiation between and within the populations. A total of ten primers used generated 146 amplicon of which 97.9% of loci detected revealed polymorphism. The Shannon’s indices (I) and Nei’s genetic diversity (h) among the studied populations from the various regions were estimated at 0.6216 (SD = 0.1199) and estimate of gene flow (Nm) in the population was 5.0555. Cluster analysis (neighbor-joining, NJ) revealed that the North-West populations (KAT) are genetically distinct from the North-central populations (SOB, KAB, KAM and KAS). Ordination by Principal Component Analysis (PCA) supported the findings of NJ. The SOB and YOB; MAI and UNI populations clustered as closely related are probably as a result
of correlation between the geographical locations and gene flow.
Description
Keywords
Dipcadi filamentosum, Genetic diversity, Polymorphism, ISSR, Population
Citation
Abdulkareem, K. A., Mustapha, O. T. & Krishnamurthy, R. (2018). Genetic diversity in populations of Dipcadi filamentosum Medik. using ISSR molecular markers. Annals of West University of Timisoara, ser, Biology, 21(1): 21- 28