Browsing by Author "Ighodalo, M."

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    Plasmodium falciparum Resistance in Selected Areas of Niger State, Nigeria: Chloroquine and Sulphadoxine/Pyrimethamine Treatment Outcome and Predictive Value of Molecular Markers
    (Centrepoint Journal (Science Edition), 2019) A, Nyamngee; Edungbola, L. D.; Edogun H. A.; Ikpe, R. T.; Agbendeh L. N.; Ighodalo, M.
    Malaria is a public health problem affecting two third of the world population with high mortality among children and pregnant women. A study of the determination of drug-resistance molecular markers in Plasmodium falciparum infection was carried out in three malaria endemic local government areas of Niger State, Nigeria between October 2018 and September 2019. The objective of the study was to determine the prevalence of molecular markers of resistance to chloroquine and sulphadoxine/pyrimethamine. P. falciparum infected red blood cells (RBC) were confirmed using microscopy, and deoxyribonucleic acid extraction from P. falciparum positive RBC specimen was done using chelex extraction method. Nested polymerase chain reaction followed by Restriction Fragment Length Polymorphisms (PCR/RFLP) were used for the detection of Plasmosdium falciparum chloroquine resistance transporter (pfcrt), P. falciparum multidrug resistance 1 (pfmdr 1), P. falciparum dihydrofolatereductase (pfdhfr) and P.falciparum dihydropteroate synthase (pfdhps). The results revealed well characterized molecular markers of P. falciparum resistance to the 4- aminoquinolines (p<0.05) while the antifolate drugs revealed high prevalence(p<0.05) of resistance: 41%, 60%, 51% and 47% of P. falciparum isolates at codons N86Y, K76T, S108N, N51I and A437G respectively. The prevalence of resistance of isolates to these antimalarial drugs were comparatively high. Therefore,