Browsing by Author "Ibrahim, Y.K.E"

Now showing 1 - 4 of 4
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    Clinical Bacterial Isolates from Hospital Environment as agent of Surgical Wound Nosocomial Infection.
    (Faculty of Pharmaceutical Sciences, University of Jos, 2010) Giwa, A; Attah, R.F; Ibrahim, Y.K.E; Olurinola, P.F; Akanbi II, A.A; Sanni, A.A
    The relationship between bacteria isolated from the hospital environment and those from wounds of operated patients was investigated to determine the causal agents of surgical site nosocomial infections. The study was carried out on bacterial species isolated from the theatre, surgical ward and patients’ surgical wounds in a tertiary health institution in Nigeria. Bacteria were isolated from the air, floor and patients’ surgical wounds in the theatre and surgical ward by using MacConkey agar, Chocolate agar Nutrient agar and Peptone water broth as isolating media. Plasmid sizes and bands of selected twenty (20) of the isolates were determined by electrophoresis analysis to determine their relatedness. The bacterial species isolated were: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Proteus mirabilis, Proteus vulgaris, Citrobacter freundii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Serratia marscenses. The result of electrophoresis showed that some of the isolates from the hospital environment and surgical sites have the same number of bands and molecular weight. It was concluded that isolates from the hospital environment with the same numbers of bands and molecular sizes with those isolated from patients wounds in the same hospital environment are of the same strain, and must have come from the same source, and therefore are likely to be responsible for the surgical wound infections observed in the patients studied.
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    Plasmid Profile of Multidrug Resistant Isolates from Water and Fish
    (Journal of pharmaceutical research, development and practice, University of Ilorin, 2018-12) Olufadi-Ahmed, Haishat Y.; Aliyu, Abdulmalik; Ibrahim, Y.K.E; David, Mosebolatan Susan; Bello, Ronke Hadiyyah
    Plasmids are extrachromosomal genetic material that can replicate independently and usually harbour antibiotic resistance genes. This study evaluated the presence and sizes of plasmid from multiple antibiotic resistant (MAR) isolates from water and fish using acridine orange as a plasmid curing agent. The minimum inhibitory concentration (MIC) of acridine orange to the 22 MAR ranged between 0.625 – 5.0 mg/mL. Majority (86.4 %) of the MAR isolates lost their resistant and became susceptible to more antibiotics indicating that their resistance was plasmid encoded. As much as 68.4 % of the isolates had one or more plasmid bands with sizes varying from 1.0 -3.0 kbp. The presence of plasmids - carrying MAR isolates in fish and river may heighten the dissemination of antibiotic resistance genes to the environment which will pose a serious threat to the human health.
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    Plasmid Profile of Multidrug Resistant Isolates from Water and Fish.
    (Journal of Pharmaceutical Research, Development & Practice, 2018-12) Aliyu, A.; Ibrahim, Y.K.E; Oyi, A. R; David, M. S; Olufadi-Ahmed, H.Y; Bello, R.H
    Plasmids are extrachromosomal genetic material that can replicate independently and usually harbour antibiotic resistance genes. This study evaluated the presence and sizes of plasmid from multiple antibiotic resistant (MAR) isolates from water and fish using acridine orange as a plasmid curing agent. The minimum inhibitory concentration (MIC) of acridine orange to the 22 MAR ranged between 0.625 – 5.0 mg/mL. Majority (86.4 %) of the MAR isolates lost their resistant and became susceptible to more antibiotics indicating that their resistance was plasmid encoded. As much as 68.4 % of the isolates had one or more plasmid bands with sizes varying from 1.0 -3.0 kbp. The presence of plasmids - carrying MAR isolates in fish and river may heighten the dissemination of antibiotic resistance genes to the environment which will pose a serious threat to the human health.
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    : Prevalence of surgical site nosocomial infection in tertiary health care institution in Nigeria
    (Seiknow publication Ltd, 2013) Atata, R. F; Ibrahim, Y.K.E; Olurinola, P.F; Adigun, I.A.; Giwa, A.; Abdul, I.F.; Akanbi, A.A.