Browsing by Author "Halimat Amin Abdulrahim"

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    Carbamazepine evoked reproductive toxicity in male Wistar rats: protective properties of Moringa oleifera leaves methanolic extract
    (Springer Science and Business Media LLC, 2020-09-25) Ganiu Jimoh Akorede; Suleiman Folorunsho Ambali; Mikail Garba Hudu; Mohammed M. Suleiman; Kolawole Yusuf Suleiman; Halimat Amin Abdulrahim; Lukman Oladimeji Raji; Isiaku AbdulMajeed
    Carbamazepine is an effective chemotherapeutic agent used to manage individual with epilepsy and trigeminal neuralgia. Chronic use of carbamazepine has been incriminated to cause reproductive disorders. The present study investigates the protective property of Moringa oleifera leaves methanolic extract on chronic carbamazepine-evoked reproductive toxicity in male Wistar rats. Forty male Wistar rats (150–260 g) were randomly separated into four groups with 10 rats, given distilled water (2 ml/kg), M. oleifera (200 mg/kg), carbamazepine (20 mg/kg) and M. oleifera accompanied with carbamazepine, after 30 min. The treatments were administered once in a day for 15 weeks via gavage. The pituitary and testis were evaluated for parameters measuring oxidative challenge, sperm characteristics and histological changes. Sex hormone concentrations were also evaluated from sera samples. The result of the phytochemical analysis shows that M. oleifera leaves extract contain total phenolics (7.8%) and flavonoids (22.23%), which are considered higher in the plant. The results also revealed that exposure to M. oleifera leaves mitigates the disruption in oxidative stress parameters, sex hormone concentration, sperm characteristics and histological changes. This study revealed that chronic carbamazepine administration evokes oxidative stress, partly involved in the alterations of concentration of sex hormones, sperm characteristics and histoarchitecture of pituitary gland and testes. Treatment with leaf extract of M. oleifera protects against the adverse reproductive consequences of long-term exposure to CBZ, due to its antioxidant property
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    Combination Therapy with Vitamin D and Metformin: A Potential Approach to Mitigate Testicular Dysfunction in Type 2 Diabetes Mellitus
    (Reproductive Sciences, 2024-09-25) Adeyemi Fatai Odetayo; Halimat Amin Abdulrahim; Adedotun Muiz Yusuf; Williams Oshetename Aromokhame; Ademola Muritala Olaitan; Mirabel Chisom Ugoji; Moses Agbomhere Hamed; Luqman Aribidesi Olayaki
    Type 2 diabetes mellitus (T2DM) is a multifactorial disease that cannot be linked to a single pathway, causing the observed heterogeneity among T2DM patients. Despite this level of heterogeneity, T2DM is majorly managed by metformin (MET) monotherapy. However, recent fndings have associated long-term metformin intake with progressive oxidative pancreatic β cell damage as the disease progresses. Hence, a signifcant number of patients treated with MET need an alternate therapy. Hence, identifying drug combinations that can efectively alleviate diferent diabetes complications would serve as a more promising therapy that can translate into active use. Hence, this study was designed to explore the possible synergistic efect of vitamin D and metformin on T2DM-induced testicular dysfunction. Thirty healthy male Wistar rats (weight: 120-150 g and age: 10 ± 2 weeks) were randomly divided into control, diabetes untreated (HFD+STZ), diabetes + vitamin D (1000 IU/kg), diabetes + metformin (180 mg/kg), and diabetes + vitamin D + metformin. All treatments lasted for 28 days and animals were sacrifced using IP injection of ketamine and xylaxine (40 and 4 mg/kg respectively). Vitamin D improved the ameliorative efect of metformin on T2DM-induced hyperglycemia and lipid dysmetabolism, accompanied by a signifcant decrease in testicular lactate dehydrogenase and lactate. Also, vitamin D + metformin signifcantly increased serum lutein- izing hormone, follicle-stimulating hormone, testosterone, and testicular 5α reductase activities. Furthermore, vitamin D improved the anti-infammatory and antioxidant efects of metformin by signifcantly decreasing T2DM-induced increase in testicular interleukin 1beta, interleukin 6, TNF-α, nitric oxide, and NF-κB and increasing T2DM-induced decrease in interleukin 10, glutathione, superoxide dismutase, catalase, GPx, and Nrf2. Vitamin D enhanced the ameliorative efect of metformin on T2DM-induced testicular dysfunction.
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    Metformin and vitamin D combination therapy ameliorates type 2 diabetes mellitus‐induced renal injury in male Wistar rats
    (Naunyn-Schmiedeberg's Archives of Pharmacology, 2024-09-30) Halimat Amin Abdulrahim; Emmanuel Aduragbemi Owootori; Adeyemi Fatai Odetayo; Joshua Damrah Bulus; Fatimoh Bolanle Jimoh; Emmanuel Oluwamuyiwa Gabriel; Iyanu Feranmi Odiete; Luqman Aribidesi Olayaki
    Diabetic kidney disease is a major microvascular diabetes mellitus (DM) complication clinically associated with a gradual renal function decline. Although metformin is a common drug for managing DM, however, monotherapy treatment with any antidiabetic drug will necessitate dosage increment since type 2 DM (T2DM) deteriorates over time due to the increasing pancreatic β-cell dysfunction and will eventually require a combination therapy approach with another antidiabetic medica- tion. Vitamin D is a food supplement that has been proven to have antidiabetic and reno-protective activities. Hence, we explore the combination of vitamin D and metformin on T2DM-induced renal dysfunction. Thirty male Wistar rats were randomized into fve (5) groups: control, diabetes untreated, diabetics treated with metformin, vitamin D, and vitamin D+metformin. Vitamin D and metformin signifcantly reversed DM-induced hyperglycemia, electrolyte imbalance, and dyslipidemia. Also, vitamin D and metformin reversed T2DM-induced increase in serum creatinine and urea and renal lactate, LDH, and oxido-infammatory response. These observed alterations were accompanied by an increase in proton pump activities and modulation of Nrf2/Nf-κB and XO/UA signaling. This study revealed that vitamin D and/or metformin ameliorated T2DM-induced renal injury.