Browsing by Author "Danja, B.A."
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Item Comparative study on the antimicrobial activity of partitioned fractions of the stem-bark of Ceiba pentandra (bombacaceae)(Nitte University, 2015) Njinga, N.S.; Sule, M.I.; Pateh, U.U.; Hassan, H.S.; Ache, R.N.; Abdullahi, S.T.; Danja, B.A.Due to the emergence of more and more drug resistance bacteria and the reported antibacterial activity of Ceiba pentandra, the antimicrobial activity of the partitioned ethyl-acetate and n-butanol fractions of the stem-bark of this plant were carried out on the following clinical isolates: Staphylococcus aureus; Streptococcus pyrogenes; corynebacterium ulcereans; Escherichia coli; Salmonella typhi; Shigella dysenterea; Enterococcus aerogenes; Pseudomonas aeruginosa; klebsiella pneumonia and the fungi Trichophytom rubrum, Microsporum sp., Aspergillus fumigatus and Aspergillus niger . Agar diffusion and broth delusion methods were used in this study. The ethyl acetate fraction showedwide spectrumantibacteria activity (with zones of inhibition between 27mmand 37mm)while the n-butanol extract showed activity only against the gram negative bacteria (zones of inhibition between 20mmand 21mm). The MIC ranged from0.65 to 2.5mg/ml and 2.5 to 5mg/ml andMBC ranged from2.5mg/ml and 5 – 10mg/ml for the ethylacetate and n-butanol respectively. Both fractions showed no activity against the fungi used in this study. The preliminary phytochemistry of the ethyl acetate showed the presence of only flavonoid and thismay explain the activity against all the bacteria. The n-butanol showed the presence of carbohydrates, saponins, tannins and cardiac glycoside and the absence of flavonoid and alkaloids. This study justifies the use of this plant in herbalmedicine.Item Phytochemical and antimicrobial activity of the leaves of lannea kerstingii engl & k. Krause (anacadiaceae)(Nitte University, 2014) Njinga, N.S.; Sule, M.I.; Hassan, H.S.; Ahmad, M.M.; Abdullahi, S.T.; Danja, B.A.; Bawa, BThe phytochemical and antimicrobial activity of the petroleum ether and crude methanol extracts, chloroform and ethyl acetate fractions of the leaves of Lannea kerstingii were investigated. Phytochemical screening revealed the presence of steroids and triterpenes in the petroleum ether extract, steroid, triterpene, flavonoids and tannins in both crude methanol extract and chloroform fraction while the ethyl acetate fraction contained only flavonoids and tannins. The extracts exhibited antimicrobial activities with zones of inhibition ranging from 17.00 to 21.03, 20.10 to 25.24, 25.32 to 34.02 and 22.28 to 27.20 mm for petroleum ether extract, methanol extract, chloroform and ethyl acetate fractions respectively. The minimum inhibitory concentration was between 5 and 10mg/ml, 5mg/ml for the petroleum ether and methanol extract respectively, and between 2.5 and 5 mg/ml, 5mg/ml for the acetate fractions. The minimum bactericidal concentration for all the extracts was 40mg/ml respectively except for chloroform fraction which ranged from 20 to 40mg/ml. The minimum fungicidal concentration for all the extracts was found to be 40mg/ml respectively. This result indicates the broad spectrum antimicrobial potential of L. Kerstingii and justifies the use of this plant in traditional medicine.