Browsing by Author "Coker, Akitoye"
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Item MOLECULAR CHARACTERIZATION OF UREAPLASMA SP. ISOLATED FROM PATIENTS WITH SEXUALLY TRANSMITTED INFECTIONS IN SELECTED CENTERS NIGERIA(PAKISTAN JOURNAL OF MEDICINE AND DENTISTRY, 2017) Olorunshola, Isaac; Coker, Akitoye; Adegboye, David; Anorlu, Rose; Banwat, Edmud; Egah, Daniel; Lombin, LamiBackground: Urea plasma species, the main human ureolytic pathogens, usually implicated in opportunistic infections. This study was aimed at comparing the sensitivity of the culture method and PCR in identifying Urea plasma species; and to evaluate the sensitivity of PCR in distinguishing Urea plasma urealyticum and Urea plasma parvum in clinical samples using a newly designed primer from our laboratory. Methods: Subjects included were both male and female patients attending STD reference centers (Lagos University Teaching Hospital and Jos University Teaching Hospital consisting of both male and female. A total of 119 patients (32 male, 87 female) who have been infertile for at least two years or those that present manifestations or reports of infertility, urethritis, spontaneous abortion, pelvic inflammatory diseases, preterm, low birth weight and neonatal pneumonia were included in this study. For the control, 117 asymptomatic male and female (w/o history or clinical symptoms of infertility) were recruited. Results: The PCR assay showed more sensitivity and specificity in the identification of Urea plasma species than culture. Of the 119 patients, 26 (21.85%) were positive for U. urealyticum, while 49 (41.18%) were positive for U. parvum and 19 (15.97%) for both organisms. Of the 117 control specimens, 15 (12.82%) were positive for U. urealyticum, while 20 (17.09%) for U. parvum and 3 (2.56%) for both organisms. Conclusion: PCR assay showed more sensitivity and specificity in the identification of Urea plasma species than culture Application of modern molecular techniques should be encouraged for the study of Urea plasma sp and other related fastidious organisms in Nigeria.Item Prevalence of EHEC O157: H7 in patients with diarrhoea in Lagos, Nigeria(APMIS-JOURNAL OF PATHOLOGY, MICROBIOLOGY AND IMMUNOLOGY, 2000-07) Olorunshola, Isaac; Smith, Stella; Coker, AkitoyeThe prevalence of sorbitol‐nonfermenting Escherichia coli O157:H7 (EHEC) was assessed in 100 patients with diarrhoea by stool culture on sorbitol MacConkey agar. The cytotoxicity of the EHEC strains was analysed by Vero cell assay and the antimicrobial susceptibility pattern of the isolates was determined. Detection rate of EHEC O157:H7 was 6%. Five of the six patients were males. Three of the isolates were from children and one was from a teenager. All strains induced cytotoxic effects in the Vero cell assay. All isolates were susceptible to most of the antimicrobials tested. The results showed that diarrhoea caused by EHEC O157:H7, a potentially life‐threatening pathogen, has remained common particularly among the child population of Lagos during the past 10 years (5). There must therefore be adequate meat and food inspection to improve the general hygiene of local fast food restaurants, so‐called ‘bukkas’, which are regarded as likely sources of infection.Item Prevalence of Human Genital Ureaplasma sp. in a Cohort of Subjects in Southern and Northern, Nigeria(Nig. J. Pure & Appl. Sci.. A (27): 3186-3191., 2018) Olorunshola, Isaac; Coker, Akitoye; Adegboye, David; Anorlu, Rose; Banwat, Edmund; Egah, Daniel; Lombin, LamiUreaplasma sp. can be found on the mucosal surfaces of the cervix or vagina of 40 to 80% of sexually matured women and implicated in several complications from urethritis to miscarriages. This study was thus aimed at ascertaining the prevalence of genital Ureaplasma sp. in Southern and Northern, Nigeria. Qualitative case controlled study was carried out in two different cities in Nigeria, while convenience sampling and a closed ended questionnaire where used to obtain data from 824 subjects. Triplicate urogenital swabs were taken from participants and transferred into transport and culture media. Specimens were inoculated onto A7 agar incubated at 37oC, for 5 days in 5% CO2. Cultures were examined microscopically daily for 5 days for the appearance of typical mycoplasma colonies. Results showed that Ureaplasma sp. was isolated from 22 of 70 males (31.4%) at the Northern Nigerian location, and from 19 of 70 males (27.1%) at the Southern Nigerian location. In females, 83 of 156 (53.2%) swabs were positive at JUTH, and 50 of 104 (48%) at LUTH. At the two locations, a total of 324 asymptomatic participants were sampled, and 28(8.6%) were positive. The prevalence rate of Ureaplasma sp. among the sexually transmitted disease (STD) patients was 24% while a value of 3.9% was recorded for the STD controls. Risk factors associated with genital Ureaplasma sp infection among the subjects include subjects with STI (p value=0.030), those with multiple number of sexual partners (p value=0.040), lack of the use of condoms (p value=0.014), age of sexual debut <18 years of age (p value=0.023) subjects with low socioeconomic status in occupation (p value=0.020), and level of education (p value=0.025). The association of genital mycoplasmas infections was strongest in participants <40 years of age (p value=0.059).