Browsing by Author "Bello, Ronke Hadiyyah"
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Item Biofilm Detection and Antibacterial Susceptibility Pattern of Isolates from In-patients with Urinary Tract Infection in a Tertiary Hospital(Journal of pharmaceutical research, development and practice, University of Ilorin, 2018-12) Aliyu, Abdulmalik; Bello, Ronke Hadiyyah; David, Mosebolatan Susan; Olufadi-Ahmed, Haishat Yetunde; Salami, Omatseye; Itodo, Ene; Kola-Mustapha, Adeola T.A survey for the detection of biofilms amongst uropathogens was conducted in a Medical ward of a Tertiary hospital in Ilorin, Kwara State. This was done to determine the prevalence of biofilm producing uropathogens among patients with CA-UTI (Catheter-Associated Urinary Tract Infection) using MTP (microtitre plate) method. Effect of media variations in the production of biofilms and the antibiogram pattern of the isolates were also determined. Out of the 50 urine samples collected and analyzed, 27 (54.0 %) yielded growth and 28 isolates were identified. Klebsiella pneumoniae was the most prevalent (42.9 %), followed by Staphylococcus aureus (35.7 %) and Pseudomonas aeruginosa was the least (21.4 %). A total of 59. 3 % of the isolates were resistant to various antibiotics used and 39.3 % of the isolates were found to be multiple antibiotic resistant. Of the 28 uropathogens isolated, 21 (75.0 %) were biofilm formers comprising of 17.9 % high biofilm formers and 57.1 % of moderate biofilm formers while 25.0 % did not form biofilm in BHIsuc. High biofilm formation was observed in Staphylococcus aureus. There was variation in biofilm formation with different media as more isolates (75. 0 %) produced biofilm in BHIsuc than in TSBglu (67.9 %). Clinical isolates of Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa have potential of biofilm production which could lead to relapse of disease condition and eventually treatment failure in CA-UTI.Item CHARACTERIZATION, ANTI-INFLAMMATORY AND ANTIMICROBIAL POTENTIALS OF A HERBAL PREPARATION(Nigerian Journal of Pharmaceutical Sciences, 2019-03) Njinga, Stanislus Ngaitad; Bakare-Odunola, M.T; Mustapha, K.B; Yahaya, T; Shittu, Abiodun; Bello, Ronke Hadiyyah; Abdullahi, S.T.; Usman, S.O.; Ayanniyi, Rashidat Oluwafunke; Olufadi-Ahmed, HaishatHerbal product (HP) (from Ricinus communis and honey) is used locally for the management of inflammatory and inflammation related disorders including asthma. The current study was carried out to determine the phytochemicals, total phenolic and flavonoid contents, chemical profile, elemental components, anti-inflammatory and antimicrobial activity of the HP used for treatment of asthma. Total phenolic and flavonoid contents were determined by the Folin-Ciocalteus reagent and AlCl3 colorimetric method respectively. The chemo-profiling was done using TLC. Elemental analysis was determined using Atomic Absorption Spectroscopy (AAS). Albumin-induced paw oedema model was used to determine the anti-inflammatory activity while agar well diffusion method was used to determine the antimicrobial activity of the HP at different concentrations. Flavonoids, saponins, alkaloid, cardiac glycoside, steroids and terpenoids were detected in the HP. Total phenolic and flavonoid contents obtained were 10.00 mg/g gallic acid equivalent and 336.60 mg/g quercetin equivalent respectively. The chemo-profiling showed one spot at 254 nm but none at 366 nm. AAS indicated the presence of K, Ca, Fe, Na, Zn, Cu and Pb (Cu and Pb were within WHO acceptable limit). The 100 % concentration produced sudden but transient significant reduction in oedema size at 30 minutes. However, at 60 minutes the effect produced at 25 % was comparable to that of Ibuprofen at 20 mg/kg body weight. At 100% concentration HP all the organisms tested were sensitive except for Pseudomonas aeruginosa. The presence of flavonoids may justify the anti-inflammatory and antimicrobial activities thus validating its use in local treatment of AsthmaItem Plasmid Profile of Multidrug Resistant Isolates from Water and Fish(Journal of pharmaceutical research, development and practice, University of Ilorin, 2018-12) Olufadi-Ahmed, Haishat Y.; Aliyu, Abdulmalik; Ibrahim, Y.K.E; David, Mosebolatan Susan; Bello, Ronke HadiyyahPlasmids are extrachromosomal genetic material that can replicate independently and usually harbour antibiotic resistance genes. This study evaluated the presence and sizes of plasmid from multiple antibiotic resistant (MAR) isolates from water and fish using acridine orange as a plasmid curing agent. The minimum inhibitory concentration (MIC) of acridine orange to the 22 MAR ranged between 0.625 – 5.0 mg/mL. Majority (86.4 %) of the MAR isolates lost their resistant and became susceptible to more antibiotics indicating that their resistance was plasmid encoded. As much as 68.4 % of the isolates had one or more plasmid bands with sizes varying from 1.0 -3.0 kbp. The presence of plasmids - carrying MAR isolates in fish and river may heighten the dissemination of antibiotic resistance genes to the environment which will pose a serious threat to the human health.