Browsing by Author "Asogwa, Nnaemeka"
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Item Hepatoprotective and Anticancer Potentials of Moringa oleifera and Musa sapientum Extracts against Cadmium Chloride Induced Hepatotoxicity in Rats(The School of Pharmacy, University of Nairobi, Nairobi, Kenya, 2022-09-01) Akinlolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbohunka, Bamidele; Arowolo, Zainab; Garuba, TaofeeqAnticancer potential of MO11 (fractionated from Moringa oleifera leaves) and MS06 (fractionated from Musa sapientum suckers) against cadmium chloride induced hepatotoxicity, demyelination, carcinogenesis, and metastasis is reported. The activity was evaluated for 17 days in 24 adult male Wistar rats randomly divided into six groups (n=4). The baseline control Group 1 received normal saline only for the entire study period. Groups 2, 3, 4 and 6 received single CdCl-dose on Day 1. Group 2 (negative control) received no further treatment, while Groups 3, 4 and 6 were treated with plant extracts MO11, MO11+MS06, and doxorubicin (positive control), respectively, on Days 1-17. Group 5 received olive oil vehicle only for the 17 days. Levels of neurotransmitters (dopamine and glutamate), and biomarkers of myelination (myelin basic protein, MBP), drug metabolism and carcinogenesis (cytochrome p450), apoptosis (caspase-3 and p53), and angiogenesis (soluble vascular endothelial growth factor receptor, sVEGFR) in liver homogenates were determined using enzyme-linked immunosorbent assay. The data were statistically analysed using Mann-Whitney U test with p ≤ 0.05. The MO11, MO11+MS06, and doxorubicin upregulated dopamine, glutamate, and cytochrome p450, but downregulated MBP, caspase-3, p53 and sVEGFR in Groups 3, 4 and 6, compared with Group 2, implying the hepatoprotective, re-myelination, and anticancer potential of the studied plant fractions.Item Hepatoprotective and Anticancer Potentials of Moringa oleifera and Musa sapientum Extracts against Cadmium Chloride Induced Hepatotoxicity in Rats(Department of Pharmaceutical Chemistry, School of Pharmacy, University of Nairobi, Nairobi, Kenya., 2021) Akinolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbohunka, Bamidele; Arowolo, Zainab; Garuba, TaofeeqAnticancer potential of MO11 (fractionated from Moringa oleifera leaves) and MS06 (fractionated from Musa sapientum suckers) against cadmium chloride induced hepatotoxicity, demyelination, carcinogenesis, and metastasis is reported. The activity was evaluated for 17 days in 24 adult male Wistar rats randomly divided into six groups (n=4). The baseline control Group 1 received normal saline only for the entire study period. Groups 2, 3, 4 and 6 received single CdCl-dose on Day 1. Group 2 (negative control) received no further treatment, while Groups 3, 4 and 6 were treated with plant extracts MO11, MO11+MS06, and doxorubicin (positive control), respectively, on Days 1-17. Group 5 received olive oil vehicle only for the 17 days. Levels of neurotransmitters (dopamine and glutamate), and biomarkers of myelination (myelin basic protein, MBP), drug metabolism and carcinogenesis (cytochrome p450), apoptosis (caspase-3 and p53), and angiogenesis (soluble vascular endothelial growth factor receptor, sVEGFR) in liver homogenates were determined using enzyme-linked immunosorbent assay. The data were statistically analysed using Mann-Whitney U test with p ≤ 0.05. The MO11, MO11+MS06, and doxorubicin upregulated dopamine, glutamate, and cytochrome p450, but downregulated MBP, caspase-3, p53 and sVEGFR in Groups 3, 4 and 6, compared with Group 2, implying the hepatoprotective, re-myelination, and anticancer potential of the studied plant fractions.Item Immunochemical Evaluation of Biomarkers of Carcinogenesis, Angiogenesis, Neuro-Cancer Interactions and Demyelination in Cadmium Chloride-Induced Testicular Toxicity in Rats.(Bulgarian Academy of Sciences, 2023) Akinlolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbohunka, BamideleCadmium is a carcinogen. Neurotransmitter-cancer interaction and tissue-innervation impact cancer survival. This study examined repro-protective and neuro-protective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in cadmium chloride (CdCl2)-induced testicular toxicity. Twenty-four adult male rats were randomly divided into 6 groups. Group 1 was control. Groups 2-4 and 6 received intraperitoneal single-dose of CdCl2 (Day 1). Groups 3, 4 and 6 were post-treated with MO11-dose, MO11+MS06-doses and Doxorubicin-dose respectively, while Group 5 received Olive Oil-dose (vehicle) from Days 1-17. Quantitative tissue enzyme-linked-immunosorbent-assays of biomarkers of carcinogenesis and neuro-cancer interaction in testicular homogenates were evaluated. Data were analysed using Mann-Whitney-U test (p≤0.05). Results showed downregulations of MBP, Caspase-3 and sVEGFR, but upregulations of Dopamine, Glutamate and Cytochrome-p450 in Groups 3, 4 and 6, compared with Group 2. Overall, CdCl2-induced testicular toxicity, angiogenesis and neurocancer interaction were ameliorated by post-treatments with MO11 and MS06.Item MO11 and MS06 ameliorated cadmium chloride-induced neuro-inflammation, hyperplasia and apoptosis via NF-kB/ Caspase-3/p53 pathway and down-regulated sVEGFR in rats(The Spanish Association of Anatomy (SAE) and Mexican Society of Anatomy, 2022-05-05) Akinlolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbounka, Bamidele; Akintunde, Temitope; Odunola, Fatimah; Osibowale, Simisola; Adepeju, MuhideenCadmium is a neurotoxin, carcinogen and a suspected agent in aetiology of Parkinson’s disease and Alzheimer’s disease (AD). Furthermore, upregulations of Caspase-3 and p53 were reported in brains of AD patients. This study evaluated the neuroprotective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in Cadmium Chloride (CdCl)-induced neurotoxicity in the cerebrum of rats. Twenty-eight adult male wistar rats (average weight of 155 g) were randomly divided into 7 groups (n = 4). Group 1 received physiological saline. Groups 2-4 and 7 received single 1.5 mg/Kg bodyweight of CdCl (i.p.) (Day 1). Groups 3-4 and 7 were post-treated with 15 mg/Kg bodyweight of MO11, 15 mg/Kg bodyweight of MO11 + 7 mg/Kg bodyweight of MSF1 and 3.35 mg/Kg bodyweight of Doxorubicin respectively (Days 1-17). Groups 5-6 received only MO11 and Vegetable Oil (vehicle) respectively (Days 1-17). Cerebral histopathology (Cresyl Fast Violet method) was evaluated in rats. ELISA evaluations of biomarkers of pro-inflammation (IL-1β, IL-6, IL-8 and NF-kB), anti-inflammation (IL-4 and IL10), apoptosis (Caspase-3 and p53), proliferation (Ki67) and angiogenesis (sVEGFR) in cerebral homogenates of rats were also conducted. Histopathological evaluations showed a high number of chromatolytic cells in Group 2, compared with Groups 1 and 3-7. Post treatments of CdCl-induced neurotoxicity with MO11 and MS06 resulted in decreased levels of IL-1β, IL-6, IL-8, NF-kB, Caspase-3, Ki67, p53 and sVEGFR, but increased levels of IL-4 and IL-10 in Groups 3-4, compared with Group 2. Therefore, MO11 and MS06 possess neuroprotective, neuroregenerative, anti-AD, anti-inflammatory and anticancer potentials.Item Protective effect of Croton zambesicus leaf extract against carbon tetrachlorideinduced cardiac toxicity in rats(Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, 2020-06-27) Ayanniyi, Rashidat Oluwafunke; Olumoh-Abdul, Hidayah Ayodeji; Ojuade, Fatimoh Idowu; Asogwa, NnaemekaBackground: The leaves of Croton zambesicus (CZ) Muell Arg. (Euphorbiaceae) are used as a spice for food and in traditional medicine for the management of several disease conditions, including hypertension and diabetes. The study was carried out to determine the cardioprotective effects of CZ leaf extract and n-butanol fraction on carbon tetrachloride (CCl4) -induced toxicity. Male albino rats were treated for 5 days with CZ extract, n-butanol fraction, and ascorbic acid. Materials and Method: On day 6, CCl4 was administered subcutaneously for 3 days to rats in Groups II-VII and treated concurrently for 3 days with aqueous leaf extract of CZ, n-butanol fraction, and ascorbic acid. Serum levels of cardiac function markers, creatine-kinase myocardial band (CK-MB) fraction, lactate dehydrogenase (LDH) were evaluated. Antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), oxidized GSH (GPx), and malondialdehyde (MDA) were also determined. Results: Aqueous leaf extract and n-butanol fraction significantly (P < 0.05) decreased serum concentration of CK-MB and LDH. In addition, there was a significant (P < 0.001) increase in activity of antioxidant enzymes (SOD, CAT, GSH, and GPx) with a significant (P < 0.05) decrease in the concentration of MDA. Conclusion: Results from this study revealed the aqueous leaf extract of CZ attenuated toxic effects of CCl4 on the heart and thus found to have a protective potential.