Protective effect of Croton zambesicus leaf extract against carbon tetrachlorideinduced cardiac toxicity in rats
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Date
2020-06-27
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Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
Abstract
Background: The leaves of Croton zambesicus (CZ) Muell Arg. (Euphorbiaceae) are used as a spice
for food and in traditional medicine for the management of several disease conditions, including
hypertension and diabetes. The study was carried out to determine the cardioprotective effects of
CZ leaf extract and n-butanol fraction on carbon tetrachloride (CCl4) -induced toxicity. Male albino
rats were treated for 5 days with CZ extract, n-butanol fraction, and ascorbic acid. Materials and
Method: On day 6, CCl4 was administered subcutaneously for 3 days to rats in Groups II-VII and
treated concurrently for 3 days with aqueous leaf extract of CZ, n-butanol fraction, and ascorbic acid.
Serum levels of cardiac function markers, creatine-kinase myocardial band (CK-MB) fraction, lactate
dehydrogenase (LDH) were evaluated. Antioxidant enzymes, superoxide dismutase (SOD), catalase
(CAT), glutathione (GSH), oxidized GSH (GPx), and malondialdehyde (MDA) were also determined.
Results: Aqueous leaf extract and n-butanol fraction significantly (P < 0.05) decreased serum
concentration of CK-MB and LDH. In addition, there was a significant (P < 0.001) increase in activity
of antioxidant enzymes (SOD, CAT, GSH, and GPx) with a significant (P < 0.05) decrease in the
concentration of MDA. Conclusion: Results from this study revealed the aqueous leaf extract of CZ
attenuated toxic effects of CCl4 on the heart and thus found to have a protective potential.
Description
Keywords
Antioxidant enzymes, aqueous leaf extract, carbon tetrachloride, cardioprotective, Croton zambesicus