Browsing by Author "Ameen, Mubarak O."
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Item Anti-Toxic Principles from Morinda lucida And Annona muricata Down-Regulated Ki67 and Multi-Drug Resistance1 Genes In Lead-Induced Hepato-Toxicity In Rats(Penerbit Universiti Sains Malaysia, 2022-11-16) Akinlolu, Adelaja A.; Omohimoria, Temitope; Oyewopo, Adeoye; Kadir, Risikat E.; Ameen, Mubarak O.Morinda lucida (ML) and Annona muricata (AM) are ethno-medicinal plants with antioxidant potentials. In addition, lead is a toxic pollutant of global health concerns. This study evaluated the effects of column chromatography-extracted ethanolic fractions of ML and AM leaves on immuno-modulations of Ki67 and multi-drug resistance1 (MDR1) proteins in the liver of rats in lead acetate (LA)-induced hepato-toxicity in-order to determine their hepato-protective, anti-proliferation, anti-drug resistance and anti-cancer potentials. Sixty adult female rats were randomly divided into 12 groups (n = 5). Groups 1 and 2 received physiological saline and 100 mg/kg bodyweight of LA, respectively, for 5 weeks. Groups 3–6 received 100 mg/kg bodyweight LA for 2 weeks followed by post-treatments with 7.5 mg/kg and 15 mg/kg bodyweight of ML, and 7.5 mg/kg and 10 mg/kg bodyweight of AM, respectively, for another 3 weeks. Groups 7–10 received 7.5 mg/kg and 15 mg/kg bodyweight of ML, and 7.5 mg/kg and10 mg/kg bodyweight of AM, respectively, for 5 weeks. Groups 11 and 12 received co-administrations of 100 mg/kg bodyweight LA simultaneously with 15 mg/kg bodyweight ML and 10 mg/kg bodyweight of AM, respectively, for 5 weeks. Drugs and extracts were administered orally. Consequently, liver histopathology (heamatoxylin and eosin technique) and enzyme linked immunosorbent assay (ELISA) homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analysed (p ≤ 0.05). Results showed normal liver histology in all groups. Post-treatments of LA-induced hepato-toxicity resulted in statistically significant (p ≤ 0.05) and non-significant decreased concentrations (p ≥ 0.05) of Ki67 and MDR1 in Groups 3–12 compared with Group 2. These observations indicated that ML and AM ameliorated LA-induced hepato-toxicity, abnormal proliferation, drug resistance and mutagenesis.Item PHYTOCHEMICAL SCREENING, ANTI-OXIDANT AND ANTI-MICROBIAL ACTIVITIES OF FRACTIONATED ETHANOLIC EXTRACT OF Moringa Oleifera LEAVE(Chemical Society of Nigeria, 2021) Ameen, Mubarak O.; Akinolu, A. A.; Garuba, Taofeeq; Ebito, G. E.; Arowolo, Zainab B; Oyebanji, E. O.Moringa oleifera leave has been reputed for the treatment of different ailments, this work thus aims at isolating the active principle(s) that are responsible for these activities. M. oleifera leave was extracted with distilled ethanol, concentrated to afford the crude extract (210.2 g). The crude extract was partitioned using n – hexane (NH), dichloromethane (DCM), ethyl acetate (EA) and methanol (MeOH) in an increasing order of polarity to afford 12 fractions. The fractions were subjected to biological testing and MOF8 and MOF11 were selected for further purification based on their antioxidant and antimicrobial activities. The two fractions (MOF8 and MOF11) were purified on a silica gel open column, using NH, DCM, EA and MeOH in an increasing order of polarity to afford ten and eight fractions respectively. Further biological test on these fractions indicates superior activities of MOF118 which was further purified as described earlier to afford four fractions. Further biological testing on these fractions indicates that and MOF118.3 and MO118.4 are more active in the antioxidant and antimicrobial analysis. Phytochemical screening of MOF11 and MOF11 indicates the presence of flavonoids, saponin, tannins, alkaloids, glycosides and steroids.Item Structural And Phytochemical Characterization of Bioactive Components of the Endophytic Fungi (Trichoderma harzianum) Extracts(University of Port Harcourt, 2022) Ameen, Mubarak O.; Garuba, Taofeeq; Zubair, M. F.; Baker, M. T.; Arowolo, Zainab B.This research aims to investigate the antioxidant activity of the crude extract of endophytic fungi (T. harzianum) and to test for the presence of phytochemicals. Fungi isolated from the leaf and stem of endemic medicinal plant were extracted with ethyl acetate. The fungi extract was then investigated for its phytochemicals, antioxidants and active compounds through LC-MS. Some of the phytochemicals present in abundance include saponins, alkaloids, flavonoids, phenols, and steroids, with tanins and cardiac glycosides also present in reasonable amounts. Those compounds identified by LC-MS with antioxidant properties include inosine diphosphate, vigabatrin, isoamylnitrite, proline, trihexyphenidyl-N-oxide, N-methyl gabapentin, penbutolol, dextromoramide M2, solanidine, aceclidine, desethyleneciproflaxin, sapropterin and kinetin.