Browsing by Author "Akolade, Jubril Olayinka"

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    Cypermethrin and chlorpyrifos raises serum urea level and causes abnormal sperm morphology in Wistar rats
    (Biointerface Research in Applied Chemistry, 2019) Iyiola, Oluyinka Ajobola; Sulaiman, A.F; Sulaiman, Abdulfattah Afolabi; Anifowoshe, Abass Toba; Akolade, Jubril Olayinka; Adisa, Muhammad Jamiu; Otohinoyi, D.A; Rotimi, Damilare E.; Batiha, Gaber El-Saber; Maimako, Rotdelmwa Filibus; Adeyemi, O.S
    Chlorpyrifos (organophosphate) and cypermethrin (pyrethroid) are insecticides, which are widely used for agricultural as well as for domestic purposes. This study investigated the toxicological effect of chlorpyrifos and cypermethrin on selected organs and tissues of male Wistar rats. Nine (9) male Wistar rats were randomly grouped into three and were orally given chlorpyrifos or cypermethrin, while the control group was given distilled water for 28 days. The results revealed a significant increase (p<0.05) in rat serum AST activity for the chlorpyrifos and cypermethrin groups. Also, there was significant elevation in serum urea following oral exposure to either chlorpyrifos or cypermethrin. Conversely, a reduction in the rat liver ALP activity for treatment with cypermethrin or chlorpyrifos was recorded. The histology results revealed that the administration of chlorpyrifos but not cypermethrin for 28 days has no significant effect on the biochemical properties and sperm morphology of the rats. Taken together, findings indicate that cypermethrin and chlorpyrifos exposure in rats predisposes to renal injury, while altering sperm morphology.
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    Lipid and Antioxidant Profile of Chitosan Bound Ethylacetate Fractions of Cocos nucifera Husk Fiber in Plasmodium berghei Infected Mice
    (NISEB Journal, 2019) Sulaiman, A. Faoziyat; Oloyede, H.O.B; Akanji, M.A; Abdulraheem, A.M.O; Akolade, Jubril Olayinka; O., Garuba Taofeeq; Onaeko, Elizabeth Tosin; Aliyu, Najeeb Olamilekan; Balogun, Olalekan Bashir
    Malaria is a parasitic disease that occurs in tropical and subtropical regions of the world. About 500 million cases of malaria occur every year, and one million people, mostly children living in sub-Saharan Africa, die as a result. This study was conducted to determine the lipid and antioxidant status of chitosan bound ethylacetate fraction of cocos nucifera husk fiber in p. berghei infected mice. Swiss albino mice were innoculated with Plasmodium berghei. The forty five mice were randomly assigned into nine groups, of 5 mice each. Administration of the Therapeutic Dose (TD) (80mg/kg) and Sub Therapeutic Dose (STD) (20mg/kg) (gotten from preliminary studies) of ethylacetate extract fraction of Cocos nucifera husk fibre coupled with chitosan and chitosan/alginate was done orally for four days post-inoculation and 0.2ml of the extract was administered. Group A served as positive control (not infected), Group B received appropriate volume of distilled water, Group C received 5mg/kg. Chloroquine (infected), Group D received80mg/kg of extract (therapeutic dose), Group E received20mg/kg of extract ( sub-therapeutic dose, infected), Group F received80mg/kg of extract + Chitosan + infected, Group G received20mg/kg of extract + Chitosan + infected, Group H received80mg/kg of extract + Chitosan/ Alginate + infected, Group I received20mg/kg of extract + Chitosan/Alginate + infected. At the end of the experimental period, selected tissues was collected, isolated and homogenized. Antioxidant (MDA, GSH and GST) and lipid profile activities (Cholesterol, HDL and LDL) were determined. The results reveal that, there was a significantly decreased in the level of the non-treated groups as compared to other treated groups and control in cholesterol, HDL and LDL level while there was significant increase in triacylglyeride level in non – treated groups compared to other test groups and control after the administered ethylacetate fraction of cocos nucifera bound to chitosan microparticles. In present study reduced glutathione (GSH), GST and lipid peroxidation product malondialdehyde (MDA) were increased significantly compared to test groups and control. These results suggest that ethylacetate fraction of Cocos nucifera husk fibre bound to chitosan microparticles may boost body’s antioxidant systems, which neutralizes the effects of free radicals and also able to reverse the change in serum lipid profile caused by malaria infection.