Lipid and Antioxidant Profile of Chitosan Bound Ethylacetate Fractions of Cocos nucifera Husk Fiber in Plasmodium berghei Infected Mice
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
NISEB Journal
Abstract
Malaria is a parasitic disease that occurs in tropical and subtropical regions of the world. About 500 million
cases of malaria occur every year, and one million people, mostly children living in sub-Saharan Africa, die
as a result. This study was conducted to determine the lipid and antioxidant status of chitosan bound
ethylacetate fraction of cocos nucifera husk fiber in p. berghei infected mice. Swiss albino mice were
innoculated with Plasmodium berghei. The forty five mice were randomly assigned into nine groups, of 5
mice each. Administration of the Therapeutic Dose (TD) (80mg/kg) and Sub Therapeutic Dose (STD)
(20mg/kg) (gotten from preliminary studies) of ethylacetate extract fraction of Cocos nucifera husk fibre
coupled with chitosan and chitosan/alginate was done orally for four days post-inoculation and 0.2ml of the
extract was administered. Group A served as positive control (not infected), Group B received appropriate
volume of distilled water, Group C received 5mg/kg. Chloroquine (infected), Group D received80mg/kg of
extract (therapeutic dose), Group E received20mg/kg of extract ( sub-therapeutic dose, infected), Group F
received80mg/kg of extract + Chitosan + infected, Group G received20mg/kg of extract + Chitosan +
infected, Group H received80mg/kg of extract + Chitosan/ Alginate + infected, Group I received20mg/kg of
extract + Chitosan/Alginate + infected. At the end of the experimental period, selected tissues was collected,
isolated and homogenized. Antioxidant (MDA, GSH and GST) and lipid profile activities (Cholesterol, HDL
and LDL) were determined. The results reveal that, there was a significantly decreased in the level of the
non-treated groups as compared to other treated groups and control in cholesterol, HDL and LDL level
while there was significant increase in triacylglyeride level in non – treated groups compared to other test
groups and control after the administered ethylacetate fraction of cocos nucifera bound to chitosan
microparticles. In present study reduced glutathione (GSH), GST and lipid peroxidation product
malondialdehyde (MDA) were increased significantly compared to test groups and control. These results
suggest that ethylacetate fraction of Cocos nucifera husk fibre bound to chitosan microparticles may boost
body’s antioxidant systems, which neutralizes the effects of free radicals and also able to reverse the change
in serum lipid profile caused by malaria infection.
Description
Based on the results obtained in this research, the administration of ethylacetate fraction of Cocos nucifera husk
fibre bound to chitosan microparticles may boost body’s antioxidant systems, which neutralizes the effects of
free radicals and was also able to reverse the change in serum lipid profile caused by malaria infection.
Keywords
Chitosan, Ethylacetate, Antioxidant, plasmodium berghei, Lipid
Citation
Sulaiman1 A. Faoziyat, Oloyede1 H.O.B., Akanji1 M. A., Abdulraheem2 A. M. O., Akolade3 Jubril Olayinka, O., Garuba4 Taofeeq, Onaeko1 Elizabeth Tosin, Aliyu1 Najeeb Olamilekan and Balogun1 Olalekan Bashir (2019). Lipid and antioxidant profile of chitosan bound ethylacetate fractions of cocos nucifera husk fiber in plasmodium berghei infected mice. NISEB Journal. Vol. 19, No. 1. Pages 1-12. March, 2019. Pages 1-9. 1595-6938/2019. Published by Society for Experimental Biology of Nigeria. Available online on: https://www.researchgate.net/publication/333192267_Lipid_and_Antioxidant_Profile_of_Chitosan_Bound_Ethylacetate_Fractions_of_Cocos_Nucifera_Husk_Fiber_in_Plasmodium_Berghei_Infected_Mice ; http://www.ojs.klobexjournals.com/index.php/nisebj.