Browsing by Author "Akinlolu, Adelaja"
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Item Extraction, isolation and evaluation of anti-toxic principles from Moringa oleifera (MOF6) and Myristica fragrans (Trimyristin) upregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats.(National Institute for Pharmaceutical Research and Development, 2020-12) Akinlolu, Adelaja; Ameen, Mubarak; Quadri, Tobilola; Odubela, Kayode; Omotoso, Gabriel; Yahya, Rahmat; Biliaminu, Sikiru; Adeyanju, Muinat; Ebito, Gabriel; Otulana, JubrilThis study evaluated the neuroprotective effects of MOF6 (isolated from Moringa oleifera leaves) and Trimyristin (isolated from Myristica fragrans seeds) on Acetylcholinesterase concentrations in cerebral cortices of rats with Sodium arsenite-induced neurotoxicity. Sixty-five adult male rats (150 g-250 g) were randomly divided into thirteen groups comprising of five rats per group. Groups 1 and 3 received physiological saline and 1 ml/200 g bodyweight of Olive oil respectively for 9 weeks. Group 2 received 20 mg/kg bodyweight of Sodium arsenite (SA) for 6 weeks and left untreated for another 3 weeks. Groups 4-5 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 5.0 and 7.5 mg/kg bodyweight of MOF6 respectively for 6 weeks. Groups 6-7 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 15 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks. Groups 8-11 received 5.0 and 7.5 mg/kg bodyweight of MOF6; 15 and 30 mg/kg bodyweight of Trimyristin respectively for 9 weeks. Groups 12-13 received 7.5 mg/kg bodyweight of MOF6 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks followed by co-administration of each extract dose with 20 mg/kg bodyweight of SA for another 3 weeks. Histological examination of cerebral cortices and biochemical analyses of Acetylcholinesterase concentrations were carried out in all rats. Computed data were analyzed using Microsoft Excel 2016 with statistical significance at p≤0.05. Histopathological evaluations revealed normal histo-architecture of cerebral cortices of all rats. Results showed statistically significant (p≤0.05) increases in Acetylcholinesterase concentrations in rats of Groups 1-10 and 12 compared with Group 2 (2.78±1.76 𝜇mole/min/g). 7.5 mg/kg bodyweight of MOF6 showed the best therapeutic and neuro-regenerative potential against SA-induced neurotoxicity. Conclusions: Our findings implied that MOF6 and Trimyristin reversed downregulation of Acetylcholinesterase concentrations in SA-induced neurotoxicity in rats; and possess neuro-protective and neuro-regenerative potentials.Item Hepatoprotective and Anticancer Potentials of Moringa oleifera and Musa sapientum Extracts against Cadmium Chloride Induced Hepatotoxicity in Rats(The School of Pharmacy, University of Nairobi, Nairobi, Kenya, 2022-09-01) Akinlolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbohunka, Bamidele; Arowolo, Zainab; Garuba, TaofeeqAnticancer potential of MO11 (fractionated from Moringa oleifera leaves) and MS06 (fractionated from Musa sapientum suckers) against cadmium chloride induced hepatotoxicity, demyelination, carcinogenesis, and metastasis is reported. The activity was evaluated for 17 days in 24 adult male Wistar rats randomly divided into six groups (n=4). The baseline control Group 1 received normal saline only for the entire study period. Groups 2, 3, 4 and 6 received single CdCl-dose on Day 1. Group 2 (negative control) received no further treatment, while Groups 3, 4 and 6 were treated with plant extracts MO11, MO11+MS06, and doxorubicin (positive control), respectively, on Days 1-17. Group 5 received olive oil vehicle only for the 17 days. Levels of neurotransmitters (dopamine and glutamate), and biomarkers of myelination (myelin basic protein, MBP), drug metabolism and carcinogenesis (cytochrome p450), apoptosis (caspase-3 and p53), and angiogenesis (soluble vascular endothelial growth factor receptor, sVEGFR) in liver homogenates were determined using enzyme-linked immunosorbent assay. The data were statistically analysed using Mann-Whitney U test with p ≤ 0.05. The MO11, MO11+MS06, and doxorubicin upregulated dopamine, glutamate, and cytochrome p450, but downregulated MBP, caspase-3, p53 and sVEGFR in Groups 3, 4 and 6, compared with Group 2, implying the hepatoprotective, re-myelination, and anticancer potential of the studied plant fractions.Item Immunochemical Evaluation of Biomarkers of Carcinogenesis, Angiogenesis, Neuro-Cancer Interactions and Demyelination in Cadmium Chloride-Induced Testicular Toxicity in Rats.(Bulgarian Academy of Sciences, 2023) Akinlolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbohunka, BamideleCadmium is a carcinogen. Neurotransmitter-cancer interaction and tissue-innervation impact cancer survival. This study examined repro-protective and neuro-protective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in cadmium chloride (CdCl2)-induced testicular toxicity. Twenty-four adult male rats were randomly divided into 6 groups. Group 1 was control. Groups 2-4 and 6 received intraperitoneal single-dose of CdCl2 (Day 1). Groups 3, 4 and 6 were post-treated with MO11-dose, MO11+MS06-doses and Doxorubicin-dose respectively, while Group 5 received Olive Oil-dose (vehicle) from Days 1-17. Quantitative tissue enzyme-linked-immunosorbent-assays of biomarkers of carcinogenesis and neuro-cancer interaction in testicular homogenates were evaluated. Data were analysed using Mann-Whitney-U test (p≤0.05). Results showed downregulations of MBP, Caspase-3 and sVEGFR, but upregulations of Dopamine, Glutamate and Cytochrome-p450 in Groups 3, 4 and 6, compared with Group 2. Overall, CdCl2-induced testicular toxicity, angiogenesis and neurocancer interaction were ameliorated by post-treatments with MO11 and MS06.Item MO11 and MS06 ameliorated cadmium chloride-induced neuro-inflammation, hyperplasia and apoptosis via NF-kB/ Caspase-3/p53 pathway and down-regulated sVEGFR in rats(The Spanish Association of Anatomy (SAE) and Mexican Society of Anatomy, 2022-05-05) Akinlolu, Adelaja; Ameen, Mubarak; Ebito, Gabriel; Asogwa, Nnaemeka; Akindele, Raheem; Fagbounka, Bamidele; Akintunde, Temitope; Odunola, Fatimah; Osibowale, Simisola; Adepeju, MuhideenCadmium is a neurotoxin, carcinogen and a suspected agent in aetiology of Parkinson’s disease and Alzheimer’s disease (AD). Furthermore, upregulations of Caspase-3 and p53 were reported in brains of AD patients. This study evaluated the neuroprotective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in Cadmium Chloride (CdCl)-induced neurotoxicity in the cerebrum of rats. Twenty-eight adult male wistar rats (average weight of 155 g) were randomly divided into 7 groups (n = 4). Group 1 received physiological saline. Groups 2-4 and 7 received single 1.5 mg/Kg bodyweight of CdCl (i.p.) (Day 1). Groups 3-4 and 7 were post-treated with 15 mg/Kg bodyweight of MO11, 15 mg/Kg bodyweight of MO11 + 7 mg/Kg bodyweight of MSF1 and 3.35 mg/Kg bodyweight of Doxorubicin respectively (Days 1-17). Groups 5-6 received only MO11 and Vegetable Oil (vehicle) respectively (Days 1-17). Cerebral histopathology (Cresyl Fast Violet method) was evaluated in rats. ELISA evaluations of biomarkers of pro-inflammation (IL-1β, IL-6, IL-8 and NF-kB), anti-inflammation (IL-4 and IL10), apoptosis (Caspase-3 and p53), proliferation (Ki67) and angiogenesis (sVEGFR) in cerebral homogenates of rats were also conducted. Histopathological evaluations showed a high number of chromatolytic cells in Group 2, compared with Groups 1 and 3-7. Post treatments of CdCl-induced neurotoxicity with MO11 and MS06 resulted in decreased levels of IL-1β, IL-6, IL-8, NF-kB, Caspase-3, Ki67, p53 and sVEGFR, but increased levels of IL-4 and IL-10 in Groups 3-4, compared with Group 2. Therefore, MO11 and MS06 possess neuroprotective, neuroregenerative, anti-AD, anti-inflammatory and anticancer potentials.Item Morinda lucida and Annona muricata reduced hepatic lipid peroxidation and promoted melatonin/TNFα/p53-mediated apoptosis in sodium arsenite-induced toxicity in rats(The Spanish Association of Anatomy (SAE) and Mexican Society of Anatomy, 2022-07-28) Akinlolu, Adelaja; Oyewopo, Adeoye; Kadir, Risikat; Ameen, Mubarak; Owoniyi, Victor; Adam, Fauzeeyah; Okeleye, ShukratArsenic-induced carcinogenesis can result in cancers of the liver in exposed organisms. This study evaluated anticancer potentials of MLF1 and AMF1 extracted from Morinda lucida and Annona muicata leaves respectively in Sodium arsenite (SA)-induced toxicity in rats. Sixty adult female rats were randomly divided into 12 groups (n = 5). Group 1 was control. Group 2 received 5-weeks administrations of 10 mg/kg bodyweight of SA. Groups 3-6 received SA-dose for 2 weeks followed by 3-weeks posttreatments with MLF1-doses and AMF1-doses respectively. Groups 7-10 received only 5-weeks administrations of MLF1-doses and AMF1- doses respectively. Groups 11 and 12 received 5-weeks co-administrations of SA-dose with highdoses of MLF1 and AMF1 respectively. Drugs/extracts were administered orally. Liver histopathology (Heamatoxylin and Eosin) and ELISA concentrations of sera Melatonin and TNF-alpha were evaluated. Malondialdehyde (thiobarbituricacid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analysed. Results showed normal liver histology in Groups 1-12. Post-treatments of SA-induced toxicity with MLF1 and AMF1 resulted in significant (P≤0.05) and non-significant decreased levels (P≥0.05) of Malondialdehyde, TNF-alpha and p53, but significant (P≤0.05) and non-significant increased Melatonin levels (P≥0.05) in Groups 3-12 compared with Group 2. MLF1 and AMF1 possess anticancer, antioxidant, pro-Melatonin, anti-inflammatory and hepato-protective potentials.