Browsing by Author "Akinlolu, A. A."
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Item Anti-Cancer and Tissue-Neuroprotective Potentials of Moringa oleifera and Musa sapientum Against In-Vivo Cadmium ChlorideInduced Skin Toxicity(Association of Anatomical Societies of Africa, 2023-08-01) Akinlolu, A. A.; Ebito, G.; Ameen, M.; Nnaemeka, N.; Akindele, R.; Fagbohunka, B.Background: Cadmium is an established carcinogen. Cadmium exposure resulted in skin carcinogenesis in rats. Furthermore, neurotransmitter-cancer interaction, tissue innervation, and angiogenesis influence cancer prognosis, metastasis, and survival. In this study, we evaluated the anticancer and neuro-protective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) against in-vivo Cadmium Chloride (CdCl2)- induced skin toxicity. Methods: Twenty-four adult male wistar rats were randomly divided into six groups (n = 4). Group 1 was control. Groups 2-4 and 6 received a single intraperitoneal administration of 1.5 mg/kg CdCl2 on Day 1. Thereafter, Groups 3, 4, and 6 were post-treated with 15 mg/kg MO11, 15 mg/kg MO11 + 7 mg/kg MS06, and 3.35 mg/kg Doxorubicin respectively (Days 1-17). Group 5 received an olive oil dose (vehicle) (Days 1-17). Skin histopathology and tissue-enzyme-linked-immunosorbent assays (Tissue-ELISA) of neurotransmitters (Dopamine and Glutamate), biomarkers of myelination (Myelin Basic Protein), drug metabolism and carcinogenesis (Cytochrome-p450), apoptosis (Caspase-3 and p53), proliferation (Ki67), and angiogenesis (sVEGFR) were evaluated in skin homogenates. Data were statistically analyzed using the Mann-Whitney-U test at P ≤ 0.05. Results: Histopathological analyses revealed normal skin histology (Groups 3 and 4), mild skin histo-alterations (Group 6), and gross skin histopathological alterations (Group 2). Tissue-ELISA analyses showed upregulations of dopamine, Glutamate and Cytochrome-p450, but downregulations of Myelin Basic Protein, Caspase-3, Ki67, p53, and sVEGFR in groups 3, 4, and 6, compared with group 2. MO11 and MS06 achieved better anticancer effects than doxorubicin. Conclusion: Overall, MO11 and MS06 possess histo-protective, neuro-protective, re-myelination, anti-proliferation, anti-angiogenesis, and anti-cancer potentials.Item Anticancer effects of Morinda lucida and Annona muricata on immunomodulations of Melatonin, tumor necrosis factor-alpha and p53 concentrations in lead acetate-induced toxicity in rats(Qassim University, 2021-07) Akinlolu, A. A.; Ameen, M. O.; Oyewopo, A. O.; Kadir, R. E.; Ahialaka, O.; Tijani, S.; Ogungbesan, O.; Bebeyi, R.; Adebayo, S.; Amoo, T.; Abdulazeez, M.Objectives: Lead poisoning accounts for about 0.6% of global burden of disease. Lead-induced toxicity is through confinement of oxidative stress in affected organs. We evaluated the effects of MLF1 (extracted from Morinda lucida leaves) and AMF1 (extracted from Annona muricata leaves) on lipid peroxidation and immunomodulations of Melatonin, tumor necrosis factor-alpha (TNF-α), and p53 proteins in lead acetate (LA)-induced toxicity in rats. Methods: Sixty adult female rats were randomly divided into 12 groups (n = 5). Groups 1 and 2 received physiological saline and 100 mg/kg bodyweight of LA, respectively, for 5 weeks. Groups 3–6 received 100 mg/kg bodyweight LA for 2 weeks, followed by treatments with 7.5 and 15 mg/kg bodyweight of MLF1, and 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 3 weeks. Groups 7–10 received 7.5 and 15 mg/kg bodyweight of MLF1, 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Groups 11–12 received co-administrations of 100 mg/kg bodyweight LA with 15 mg/kg bodyweight MLF1 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Drugs and extracts were administered orally. Consequently, liver histopathology (Hematoxylin and Eosin), sera Melatonin, and TNF-α (enzyme-linked immunosorbent assay [ELISA]) levels were evaluated. Malondialdehyde (MDA) (thiobarbituric acid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analyzed (P ≤ 0.05). Results: Results showed normal liver histology in all Groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased levels (P ≥ 0.05) of MDA, TNF-α and p53 in Groups 3-12, compared with Group 2. Furthermore, results showed significant (P ≤ 0.05) and non-significant increased Melatonin levels (P ≥ 0.05) in Groups 4–12 compared with Group 2. Conclusion: This study confirmed that MLF1 and AMF1 confer a degree of antioxidant, anticancer and hepato-protetive potentials against LA-induced toxicity in rats.Item The effects of Moringa Oleifera on lipid profile status, heart histology, and liver histochemistry in adult Wistar rats(Wolters Kluwer - Medknow, 2017-04-10) Akinlolu, A. A.; Bayode, E. O.; Ghazali, K. O.; Ameen, M. O.Background: Moringa oleifera’s leaves are globally used for nutritional and medicinal purposes. We evaluated lipid profile status, heart and liver histology, and the activities of alanine and aspartate transaminases of the liver in M. oleifera‑treated adult Wistar rats. Materials and Methods: Twenty‑four adult male Wistar rats were employed in the study. Rats of control Group I received physiological saline while rats of Groups II–IV received 250, 500, and 750 mg/kg bodyweight of methanolic extract of M. oleifera, respectively, for 21 days. Results: No gross morphological or behavioral anomalies were observed in rats of Groups I–IV. Histological examinations showed normal histoarchitecture of the heart in Group I and the liver of Groups I–III. Comparative histological analyses showed dose‑dependent increased nucleated cells in heart tissues of rats of Groups II–IV than those of Group I. Furthermore, histological evaluations showed hypertrophy of central vein of the liver in rats of Group IV when compared to Group I. Statistical analyses showed dose‑dependent non significant higher mean levels (P > 0.05) of total cholesterol and triglycerides in the sera of rats of Groups II–IV compared to rats of Group I. Evaluations of levels of alanine and aspartate transaminases showed a statistically significant higher mean values (P < 0.05) in Group II, but statistically nonsignificant higher mean values (P > 0.05) in Groups III–IV compared to Group I. Conclusions: No significant adverse effects on lipid profile status, heart histology, and liver histochemistry were observed in M. oleifera‑treated rats.Item Moringa oleifera and Musa sapientum ameliorated 7,12-Dimethylbenz[a]anthracene-induced upregulations of Ki67 and multidrug resistance 1 genes in rats(Qassim University, 2021-05) Akinlolu, A. A.; Oyewopo, A. O.; Kadir, R. E.; Lawal, A.; Ademiloye, J.; Jubril, A.; Ameen, M. O.; Ebito, G. E.Objectives: Moringa oleifera (MO) and Musa sapientum (MS) are plants of ethnomedicinal importance. We evaluated the effects of MOF6 (extracted from MO leaves) and MSF1 (extracted from MS suckers) on immunomodulations of Ki67 (proliferation biomarker) and multidrug resistance 1 (MDR1) genes in the liver of rats in 7,12-Dimethylbenz[a]anthracene (DMBA)-induced hepatotoxicity and mutagenesis to determine their antiproliferation, anti-drug resistance, and anticancer potentials. Methods: Forty-five adult male rats were randomly divided into nine groups (n = 5). Groups 1 and 2 received physiological saline and 15 mg/kg bodyweight of DMBA, respectively. Groups 3 and 4 received 15 mg/kg bodyweight DMBA and were treated with 15 and 30 mg/kg bodyweight of MOF6, respectively. Group 5 received 15 mg/kg bodyweight DMBA and was treated with 10 mg/kg bodyweight of MSF1. Group 6 received 15 mg/kg bodyweight DMBA and was treated with 3.35 mg/kg bodyweight of doxorubicin and intravenous injection of 0.5 ml/200 g of cisplatin. Groups 7–9 received only 15 and 30 mg/kg bodyweight of MOF6 and 10 mg/kg bodyweight of MSF1, respectively. DMBA, doxorubicin, and extracts doses were administered orally. The duration of our experimental procedure was 8 weeks. Consequently, liver histopathology (hematoxylin and eosin technique) and enzyme-linked immunosorbent assay homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analyzed (P ≤ 0.05). Results: Results showed normal histoarchitectures of the liver in all groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased concentrations (P ≥ 0.05) of Ki67 and MDR1 in Groups 3–9 compared with Group 2. Therefore, MOF6 and MSF1 ameliorated DMBA-induced hepatotoxicity, abnormal proliferation, and drug resistance. Conclusion: MOF6 and MSF1 possess antiproliferation, anti-drug resistance, and anticancer potentials.Item Moringa oleifera Impairs the Morphology and Functions of the Kidney in Adult Wistar Rats(Anatomical Society of Chile, 2014-09-10) Akinlolu, A. A.; Ghazali, O. K.; Ameen, Mubarak; Oyebanji, S. C.; Omotoso, G. O.; Enaibe, B. U.We tested the hypothesis that Moringa oleifera impairs the morphology and functions of the kidney in rats. Twenty four adult male Wistar rats were employed in the study. Rats of Control Group I received physiological saline while rats of Groups II – IV received 250, 500 and 750 mg/kg body weight of methanolic extract of Moringa oleifera respectively for twenty one days. No behavioral anomalies were observed in rats of Groups I – IV. Rats of Control Group I gained statistically significant increased body weight while rats of Groups II – IV experienced non-significant decreased body weight during experimental procedure. (P£0.05). No statistical significant differences (P£0.05) were observed in the analyses of the relative weights of kidneys of rats of Groups I – IV. Histological examinations showed normal cyto-architecture of the kidneys of rats of Group I while the Capsular spaces of the kidneys of rats of Groups II – IV appeared wider than those of Group I. Statistical analyses showed significant higher levels (P£0.05) of Alanine and Aspartate Transaminases, and serum urea in rats of Groups II – IV in a non- dose-dependent manner when compared to rats of Group I. Our findings are consistent with the stated hypothesis.Item Research Journal of Health Sciences(2018-02-01) Omotoso, G. O.; Kadir, R. E.; Gbadamosi, I. T.; Akinlolu, A. A.; Adunmo, G. O.; Kolo, R. M.; Lawal, M. O.; Ameen, M. O.Objectives: Cuprizone is a neurotoxicant used in modeling demyelinating disorders. This study explored the effects of Moringa oleifera (MO) on oxidative, histomorphological and behavioural changes in cuprizone-damaged cerebellum. Methods: Twenty adult female Wistar rats were grouped into 4, each group having five animals. Group A received 1 ml of normal saline (Control); group B received 0.4% cuprizone; group C received 15.6 mg/kgBW Moringa oleifera leaf extract; group D received 0.4% cuprizone and 15.6 mg/kgBW Moringa oleifera, orally for 5 weeks. The animals were assessed for exploratory and locomotor activities, while the cerebellum was processed for histology and assayed for nitric oxide (NO), catalase (CAT) and superoxide dismutase (SOD) activities. Results: Cuprizone treatment caused weight reduction, disruption of Purkinje cell layer, cellular degeneration, reduction in NO, CAT and SOD activities. However, these changes were ameliorated when co-administered with MO. Conclusion: The anti-oxidative property of Moringa oleifera is responsible for its ameliorative effect in cuprizone neurotoxicity.Item Spondias mombin promotes gastric mucosa and lipid profile status in gastric ulceration(Brazilian Society of Anatomy and Pan-American Association of Anatomy, 2014-08-10) Akinlolu, A. A.; Ghazali, K. O.; Ameen, Mubarak; Odewabi, A. O.Introduction: Spondias mombin has been reported to possess medicinal properties. Material and Methods: This study evaluated the anti-ulcer properties of Spondias mombin. Rats of Group I received physiological saline only while 40mg/kg/bodyweight of Indomethacin (an ulcerogen) was administered to rats of Groups II – VI. Four hours after administrations of the ulcerogen; rats of Groups III – VI were treated daily with oral administrations of 250, 500 and 1000mg/kg/bodyweight of Spondias mombin bark extract and 40mg/kg/bodyweight of Omeprazole respectively for four days. Rats were euthanized on Days 1 (Group II) and 5 (Groups I and III – VI); stomach and liver samples were removed for evaluations of gastric acidity, histo-pathological and lipid profile status. Results: Analyses of gastric acidity and lipid profile status in the stomach and liver samples of rats of Group II showed statistically significant higher levels (P≤0.05) of gastric acidity, total cholesterol, low density lipoprotein-cholesterol and triglycerides but lower high density lipoprotein-cholesterol levels (P≤0.05) when compared to rats of Groups I, and III – VI. Histopathological evaluations showed dose-dependent restorations of stomach and liver histology to pre-ulceration states in rats of Groups III – VI. Conclusions: This study concluded that Spondias mombin administrations promoted histo-pathological restorations of the stomach and liver; and lipid profile status of male wistar rats in Indomethacin – induced gastric ulceration.