Honey reverses disease progression and has anti-nociceptive and anti-inflammatory effects in a rat model of knee osteoarthritis induced by monosodium iodoacetate.
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Date
2021-01-21
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Elsevier
Abstract
Background: Clinically, osteoarthritis manifests as joint pain with
concomitant loss of joint function, which may ultimately result in a
substantially reduced quality of life for the patient. Although, a lot
is known about the symptom of the disease, the pathophysiology
behind the structural changes is complex and poorly understood.
By understanding the mechanisms driving joint tissue destruction
in osteoarthritis and identifying the key factors involved, new
targets for therapy are emerging that will go beyond symptomatic
relief to slowing or stopping the progression of osteoarthritis.
Aim: Thus, the aim of this study was to evaluate the effect of honey
on disease progression, pain perception and inflammation in
monosodium iodoacetate (MIA)-induced knee osteoarthritis in
female Wistar rats.
Methods: Thirty, twelve-month old female Wistar rats, weighing
between 200 g & 250 g, were randomly divided into five groups of
six animals each. Animals in group one were not induced and
served as the control, while animals in groups two to five were
injected with monosodium iodoacetate in the right knee. In
addition, animals in group two received normal saline (1 ml/kg
b.w.), group three received arthocare (glucosamine/chondroitin
sulphate 6.67/8.33 mg/kg b.w.), group four received low dose
honey (250 mg/kg b.w.) while group five received high dose honey
(1,000 mg/kg b.w.) and were treated for twenty one days. All animals
were subjected to assessment of tactile allodynia (von Frey
test), acute inflammation (knee edema), and serum biomarkers:
tumour necrosis factor-alpha (TNF-a), vascular endothelial growth factor (VEGF), prostaglandin E2 (PG E2) & cartilage oligomeric
matrix protein (COMP) as well as histo-pathological assessment of
the right knee joint.
Results: Honey (at high and low doses), significantly (p < 0.05)
reduced tactile allodynia on von Frey test (60 ± 20g) in animals
subjected to experimental knee osteoarthritis induced by MIA.
Knee edema was also significantly (p < .05) reduced by both high
and low doses of honey. Low dose honey significantly (p < .05)
reduced he serum levels of TNF-a (61.5 ± 22 pg/ml), VEGF
(31 ± 6.1 pg/ml) and COMP (41 ± 14 ng/ml) but, had no effect on
the serum level of PG E2. High dose honey on the other hand, only
significantly reduced the serum level of TNF-a (87 ± 22 pg/ml) but,
had no effect on the serum levels of VEGF, COMP and PG E2.
However, the administration of honey did not show any significant
effect on histo-pathological features of the induced osteoarthritis.
Conclusion: Honey reversed disease progression and reduced pain
perception as well as inflammation in MIA-induced knee osteoarthritis
in female Wistar rats. However, honey had no effect on
the histo-pathology of the knee joint.
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Citation
Honey, Monosodium iodoacetate, Osteoarthritis, Pain, Rat