Sodium acetate ameliorated systemic and renal oxidative stress in high-fructose insulin-resistant pregnant Wistar rats
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Date
2021-02-27
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Publisher
Springer
Abstract
Pregnancy is an insulin-resistant condition, especially at near term predisposing maternal kidneys to hyperinsulinemia-induced
oxidative stress. The impact of fructose on renal metabolic dysregulation and oxidative stress in pregnancy requires elucidation.
Short-chain fatty acids (SCFAs) are known for protective roles in oxidative stress conditions. Therefore, the study aimed at
investigating fructose-induced glucose dysregulation and renal oxidative stress in pregnant and non-pregnant rats and the
possible preventive role of SCFA, acetate. Thirty female Wistar rats were grouped (n = 5/group). Three groups were made
pregnant (P); the other three remained non-pregnant (NP). Both pregnant and non-pregnant rats received drinking water (control),
10% fructose (w/v) (NP+F or P+F), and 10% (w/v) fructose plus sodium acetate (200 mg/kg) (NP+F+A or P+F+A) for 3 weeks.
Renal and plasma glutathione antioxidant index (GSH/GSSG), G6PDH, and adenosine were significantly lower in NP+F and P+
F groups compared with control while renal and plasma adenosine deaminase (ADA), xanthine oxidase (XO), uric acid (UA),
lactate dehydrogenase (LDH), and malonaldehyde (MDA) were significantly elevated in NP+F and P+F groups compared with
controls. HOMA-IR showed marked impairment in both NP+F and P+F groups. The P+F group revealed greater suppression in
plasma and renal G6PDH-dependent antioxidant index, adenosine, and aggravation of LDH, MDA compared with the NP+F
group (p < 0.05). Sodium acetate reduces plasma and renal surrogate oxidative stress markers, improved G6PD-dependent
antioxidant index, and HOMA-IR in NP+F and P+F groups. Pregnancy exacerbates fructose-induced insulin resistance and
renal oxidative stress whereas acetate ameliorated fructose-induced redox and glucose dysregulation in pregnant and non pregnant rats.
Description
Keywords
Sodium acetate, Glucose dysregulation, Pregnancy, Oxidative stress, Fructose
Citation
Oyabambi, A. O., Michael, O. S., Areola, E. D., Saliu, S. B., & Olatunji, L. A. (2021). Sodium acetate ameliorated systemic and renal oxidative stress in high-fructose insulin-resistant pregnant Wistar rats. Naunyn-Schmiedeberg's archives of pharmacology, 394(7), 1425–1435. https://doi.org/10.1007/s00210-021-02058-6