Occurrence and anti – fungal Sensitivity Testing of Candida Species isolated from Canine Conjunctivitis
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Date
2016-11-21
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Publisher
International Journal of Veterinary Sciences Research.
Abstract
Fungal infection in dogs and other domestic pets caused by Candida species have been previously reported. Several
available antifungal drugs for treatment of yeast associated infections have been used with diverse effects. This study
establishes antifungal susceptibility profile on identified Candida species isolates associated with canine
conjunctivitis using disks diffusion method as described by NCCLS (M44-A). The antifungal disks used include
Amphotericin-B (20mcg), Nystatin (100units), Itraconazole (10mcg), Ketaconazole (10mcg), Fluconazole (25mcg).
The identified Candida species were C. albicans, C. krusei, C. tropicalis and C. parapsilosis. Percentage
susceptibility was high in both Amphotericin B (77.27%) and Nystatin (58.18%), while Itraconazole showed
(54.54%), Ketoconazole (55.45%) and Fluconazole was least (11.82%). The sensitivity ranking showed
Amphothericin B > Nystatin > Itraconazole > Ketoconazole > Fluconazole and increased resistance amongst the
Azoles especially Fluconazole (70.90%) in comparison with Polyenes. C. albicans showed the highest resistance
(84.51%) to both Fluconazole and ketoconazole (67.61%). C. parapsilosis showed 63.64% and 61.54% percentage
resistance to Nystatin and Itraconazole respectively. In conclusion, this finding showed that Amphotericin B and
Nystatin were most effective against isolated Candida species. Proper diagnosis of the underlying ailment is
necessary in conjunction with routine anti-fungal susceptibility testing to avoid drug abuse and resistance.
Description
Keywords
nti-fungal sensitivity testing, Candida species, Canine conjunctivitis