Sildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats

Simple item page
dc.contributor.authorAreola, Emmanuel Damilare
dc.contributor.authorSabinari, Isaiah Woru
dc.contributor.authorUsman, Taofeek Olumayowa
dc.contributor.authorAbayomi, Faith Ifeoluwa
dc.contributor.authorOnyezia, Onyeka
dc.contributor.authorOnaolapo, Bisola
dc.contributor.authorAdetokunbo, Phebe Oluwaseun
dc.contributor.authorAdebanjo, Olympus Oyewole
dc.contributor.authorOladipupo, Funmilayo Rebecca
dc.contributor.authorOlatunji, Lawrence Aderemi
dc.date.accessioned2025-05-09T11:05:46Z
dc.date.available2025-05-09T11:05:46Z
dc.date.issued2021-07-12
dc.description.abstractLeptin and hypothalamic-adipose lipid handling are relevant in determining the shift of metabolic activities. There are scanty findings connecting glucose dysregulation as a result of hyperandrogenism during gestation to hypothalamic-adipose axis and leptin resistance. Sildenafil has recently gained attention in the prevention of intra-uterine growth restriction. The present study aimed at demonstrating the effect of sildenafil on leptin resistance and hypothalamic-adipose lipid handling in testosterone-exposed pregnant rats. Three groups of pregnant Wistar rats (n ¼ 5/group) received olive oil (Ctr, S.C.) or testosterone propionate (Tes, 3.0 mg/kg; sc)or testosterone propionate (3.0 mg/kg; sc) and sildenafil (Tes þ PDE5, 50 mg/kg; po)from gestational day 14–19. Blood samples, hypothalamus and adipose tissue were excised for biochemical analysis on day 20. Adipose and body weights, plasma leptin and adiponectin, adipose and hypothalamic leptin and triglyceride, adipose uric acid, hypothalamic Nrf2, catalase and nitric oxide were reduced following gestational testosterone exposure. Also, fasting insulin, plasma triglyceride, uric acid, leptin-adiponectin ratio, hypothalamic free fatty acid, total cholesterol, uric acid, aspartate transaminase and cyclic guanine monophosphate were elevated by testosterone exposure to pregnant animals. Sildenafil ameliorated leptin resistance and normalized hypothalamic-adipose lipid handling. Therefore, sildenafil protects against testosterone-induced leptin resistance and adverse hypothalamic adipose lipid handling in pregnant rats.
dc.description.sponsorshipThis research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
dc.identifier.citation14.Areola E.D., Sabinari, I.W., Usman, T.O., Abayomi, F.I., Onyezia, O., Onaolapo, B., Adetokunbo, P.O., Adebanjo, O.O., Oladipupo, F.R., & Olatunji, L.A. (2021). Sildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats. Heliyon, 7 (7), e07574, Published by Elsevier. Available online at: Issue: Heliyon
dc.identifier.uri10.1016/j.heliyon.2021.e07574
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/123456789/16547
dc.language.isoen
dc.publisherElsevier, Cell Press
dc.relation.ispartofseries7(7); e07574
dc.subjectHypothalamus
dc.subjectAdipose tissue
dc.subjectLeptin resistance
dc.subjectPDE5 inhibitor
dc.subjectLipid handling
dc.titleSildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Paper 14.pdf
Size:
484.77 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections