Anatomical and molecular characterization of some rhigonematid parasites of millipedes in Nigeria, with new insights into their phylogeny
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Date
2023-06
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Helminthology Cambridge University
Abstract
Parasitic nematodes of millipedes from Nigeria are molecularly characterized for the first time.
During nematode surveys on live giant African millipedes from several localities in Nigeria,
4 species of rhigonematids were identified by application of integrative taxonomical approaches
(morpho-anatomy and molecular markers), including Brumptaemilius sp., Gilsonema gabonensis, Obainia pachnephorus, and Rhigonema disparovis. The results of morphometric and
molecular analyses of D2-D3 28S, ITS, partial 18S rRNA, and cytochrome oxidase c subunit
1 (COI) gene sequences further characterized the rhigonematid species, and clearly separated
them from other related species. Phylogenetic relationships based on 28S and 18S rRNA genes
suggest that genera within Ransomnematoidea (Ransomnema, Heth, Carnoya, Brumptaemilius,
Cattiena, Insulanema, Gilsonema) and Rhigonematoidea (Rhigonema, Obainia, Xystrognathus,
Trachyglossoides, Ichthyocephaloides) clustered rather closer than could be expected in view of
their morphological differences. Phylogenetic relationships based on ITS and COI are congruent
with those of other ribosomal genes; however, they are not conclusive due to the scarcity of
available sequences of these genes for these genera in NCBI
Description
Phylogeny; Taxonomy
Keywords
Africa; cytochrome oxidase c subunit 1; ITS rRNA; D2-D3 of 28S rDNA; phylogeny, Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Soil science, Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Plant production::Plant and forest protection
Citation
Fabiyi OA, Bello TT, LiĆ©banas G, Clavero-Camacho I, CantalapiedraNavarrete C, Archidona-Yuste A, PalomaresRius JE, Hunt DJ, Castillo P (2023). Anatomical and molecular characterization of some rhigonematid parasites of millipedes in Nigeria, with new insights into their phylogeny. Journal of Helminthology, 97, e47, 1ā17 https://doi.org/10.1017/S0022149X23000275