Amylase activity of a yellow pigmented bacterium isolated from cassava waste
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Date
2009
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Published by Academic journals.
Abstract
This study investigated the amylase activity of a yellow pigmented bacterium isolated from cassava
wastes obtained from a dumpsite near a gari processing factory in Ibadan, Nigeria. Isolate was grown in
nutrient broth containing 1% starch and then centrifuged at 5,000 rpm. Amylase activity was assayed
using the DNSA method for the detection of reducing sugar. Best enzyme activity was recorded at 30ºC
and pH 7.5. The amylase was found to be stable after exposure to temperatures between 20 and 40ºC
and pH 6.0 to 9.0, outside which there was a decline in activity. The ability of the isolate to produce
amylase explains its colonization of a cassava waste dumpsite. Conditions of activity and stability also
agree with the growth condition of the isolate.
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Keywords
Cassava waste, yellow pigmented bacterium, amylase
Citation
Saliu B.K. (2009). Amylase activity of a yellow pigmented bacterium isolated from cassava waste. African Journal of Biotechnology. 8(20): 5487 – 5492, https://www.ajol.info/index.php/ajb/issue/view/8124