Biochemical changes in alloxan-induced diabetes in rat liver and kidney dosed artesunate.
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Date
2019
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Publisher
International Institute of Science and Technology
Abstract
Diabetes is a disease marked by elevated blood sugar levels, and the second leading cause of renal diseases and
blindness worldwide. Artesunate is an antimalarial drug, that has been reported to have hypoglycemic potential,
but to the best of our knowledge, much work has not been done to explore the biochemical and clinical
implications of administration of artesunate on a diabetic subject. This study investigates biochemical changes in
terms of oxidative status associated with oral administration of artesunate on diabetic animal model. Twenty
eight male Wistar rats weighing averagely 200g were divided into four groups of seven rats each, Group Acontrol,
B- Diabetes only, C- Artesunate only, D- Diabetes + Artesunate. Diabetes was induced intraperitoneally,
at a single dose of Alloxan(160mg/kg body weight(bw). Artesunate was administered orally in aqueous solution
at 2.90mg/kg bw on day one, and at 1.45mg/kg bw on the subsequent 7days. Spectrophotometric technique was
used for biochemical analysis in serum, kidney and liver homogenates. Aspartate amino transferase (AST) and
Alanine amino transferase (ALT) activities as well as Creatinine concentration were significantly (P<0.05)
increased in group B compared with control, while group D showed a significant (P<0.05) decrease compared
with group B. Total protein concentration was significantly(P<0.05) increased in group B compared with control,
while group D showed an insignificant decrease compared with group B. Moreover, Superoxide
dismutase(SOD) and Catalase(CAT) activities as well as Reduced glutathione (GSH) concentration were
significantly(P<0.05) decreased in group B compared with control, and were restored near to the control level
significantly (P<0.05) in group D, compared with group B. Malondialdehyde (MDA) concentration was
significantly (P<0.05) increased in group B compared with control, but was reversed significantly (P<0.05) in
group D compared with group B. Artesunate ameliorates oxidative stress in diabetic rats liver and kidney,
possess hypoglycemic effect, hence antioxidant and hypoglycemic effects of artesunate may be beneficial to
diabetic liver and kidney.