Epipremnum aureum (Linden and André) G. S. Bunting (Araceae): Free Radicals Scavenging, Cell Growth Inhibition and Cytotoxic effects on Rhabdomyosarcoma and Laryngeal Carcinoma Cancer Cells
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Date
2023
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Pharmaceutical Journal of Kenya
Abstract
Patients with chronic health conditions such as cancer often benefit from plant based herbal recipes with minimal adverse effects. There also exists growing worries over the safety and efficacy of most of the currently used conventional chemotherapeutic agents. This study examined the radical scavenging and cytotoxicity of E. aureum traditionally employed for the management of cancerous diseases.
The aerial part of Epipremnum aureum was collected from the University of Ibadan botanical garden and authenticated at the Forest Herbarium Ibadan. Air-dried aerial part of Epipremnum aureum was pulverized and extracted with 70% aq. methanol and concentrated in vacuo. A portion the methanol extract was fractionated and crude alkaloidal extract was prepared from another portion of the methanol extract. The extracts and fractions were subjected to; antioxidant DPPH radical scavenging assay and cytotoxicity of the extracts and fractions against rhabdomyosarcoma (Rd) and laryngeal carcinoma (Hep-2C) cancer and non-cancerous (Vero) cell lines were determined.
Ethyl acetate fraction and alkaloidal extract displayed better DPPH radical scavenging activity than the methanol extract and other fractions. The alkaloidal extract (CC50=0.17±0.03μg/mL) and dichloromethane fraction (CC50=0.28±0.05 μg/mL) displayed the highest cytotoxicity against Rd cancer cell lines compared to cyclophosphamide (CC50=2.23±0.33 μg/mL). However, the alkaloidal extract displayed the least cytotoxicity to Vero cell line and it is about 14.25 and 22.18 times more cytotoxic to Rd and Hep-2C cancer cells, respectively.
Key words: Radical Scavenging, Cytotoxicity, GC-MS Analysis, Epipremnum aureum
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Salawu, K. M., Ogbole, O. O., Abiodun, O. O., Mohammed, A., Abdullahi, A. A., & Ajaiyeoba, E. O. (2023), Epipremnum aureum (Linden and André) G. S. Bunting (Araceae): Free Radicals Scavenging, Cell Growth Inhibition and Cytotoxic effects on Rhabdomyosarcoma and Laryngeal Carcinoma Cancer Cells. Pharmaceutical Journal of Kenya, 27 (1), 26-32.