Metal Displacement Effects on Monoesterase Activity of Calf Intestinal Alkaline Phosphatase

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dc.contributor.authorIgunnu, Adedoyin
dc.contributor.authorArise, R. O.
dc.contributor.authorAdebayo, J. O.
dc.contributor.authorMalomo, S. O.
dc.date.accessioned2020-05-22T22:29:40Z
dc.date.available2020-05-22T22:29:40Z
dc.date.issued2012
dc.description.abstractThe mechanism of modulation of alkaline phosphatase activity by metal ions has not been fully elucidated. We investigated the time-dependent modulatory effects of Mg 2+ and Zn 2+ in promoting the hydrolysis of para-nitrophenyl phosphate (monoesterase reaction) by calf intestinal alkaline phosphatase (CIAP) and the effects of addition of the activating metal ions to the metal-inhibited enzyme. The CIAP was affected by changes in pre-incubation time in the presence of the two metal cofactors. Both Mg 2+ (0.1 – 0.25 mM) and Zn 2+ (0.1 – 5 mM) modulated Zn 2+ - and Mg 2+ - inhibited monoesterase activity of CIAP. The CIAP activity was inhibited when the enzyme was pre-incubated with 1 mM Ca 2+ . Further addition of Mg 2+ (0.1 – 2 mM) did not completely restore the activity though partly relieved the inhibition caused by Ca 2+ - pre-incubated enzyme. Again, addition of 2mM Zn 2+ to Ca 2+ - pre-incubated alkaline phosphatase completely restored the activity of the enzyme. This study suggests that Mg 2+ and Zn 2+ regulate the catalytic property of each other and modulate the inhibitory effect of Ca 2+ in alkaline phosphatase catalysis through a displacement effect. The modulation of Ca 2+ -inhibited CIAP activity by Mg 2+ and Zn 2+ may be explored in the treatment of disorders of bone mineralization especially those arising from inhibited alkaline phosphatase activity.en_US
dc.description.sponsorshipSelf Sponsoreden_US
dc.identifier.issn0189-4757
dc.identifier.urihttp://hdl.handle.net/123456789/3958
dc.language.isoenen_US
dc.publisherNigerian Society of Biochemistry and Molecular Biologyen_US
dc.relation.ispartofseriesNigerian Journal of Biochemistry and Molecular Biology;Vol. 27, Issue 1
dc.subjectAlkaline phosphataseen_US
dc.subjectmonoesterase reactionen_US
dc.subjectmetal ion cofactorsen_US
dc.subjectdisplacement reactionen_US
dc.titleMetal Displacement Effects on Monoesterase Activity of Calf Intestinal Alkaline Phosphataseen_US
dc.typeArticleen_US

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