PREPARATION OF YEAST (SACCHAROMYCES CEREVISIAE) BIOMASS FROM SUGARCANE BAGASSE
No Thumbnail Available
Date
2019-08-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Faculty of Engineering, University of Maiduguri, Maiduguri, Nigeria.
Abstract
In this study, the pretreatment by milling, dilute-acid hydrolysis of
sugarcane bagasse, and subsequent fermentation of its glucose product
was performed to investigate the effects of process conditions on the
production of Saccharomyces cerevisiae. The hydrolysis was carried out
using HCl at three substrate concentrations of 20, 25, and 30% (w/v) of
bagasse to distilled water. Hydrolysis parameters (time, acid
concentration, and temperature) were varied for each case of substrate
concentration in full factorial experiments, and an optimum glucose
yield of 1.907 g/L was obtained with the 20% (w/v) substrate
concentration, at conditions of 10 min time, 0.5 M acid concentration,
and 8 0°C temperature. Thereafter, fermentation experiment was
performed with S. cerevisiae in the product of hydrolysis. An optimum
ca. 207 yeast number of colonies (yield: 20, 700, 000 cfu/ml) was
achieved in 40 h, and the growth of S. cerevisiae was governed by the
kinetic equation, ln Xt = 8.4338 + 0.2943t.
Description
Keywords
Saccharomyces cerevisiae Sugarcane bagasse Acid hydrolysis Fermentation
Citation
5