Bioefficacy of leaf extracts of Azadirachta indica and vernonia amygdalina and their mixture against Colletotrichum falcatum
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Date
2020-01-17
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Publisher
West University of Timisoara, series Biology.
Abstract
Chemical control of red rot disease of sugarcane with synthetic fungicides, though effective, enables the pathogen to produce new pathological races or strains that are resistant to the available synthetic fungicides in the market. The synthetic fungicides, in addition, have harmful side effects on humans, other animals and the environment. This study evaluated the in vitro antifungal activity of leaf extracts of Azadirachta indica and Vernonia amygdalina against Colletotrichum falcatum, the pathogen of red rot disease of sugarcane. Pour Plate method was used to determine the effect of 10, 20 and 30 g/100 ml concentrations of aqueous and ethanolic leaf extracts of A. indica and V. amygdalina and their integration on mycelial radial growth of C. falcatum. The aqueous and ethanolic leaf extracts of the botanical species and their integrated leaf extracts inhibited the mycelial growth of C. falcatum in vitro, with the ethanolic extracts being more potent at some of the concentrations tested against the pathogens. The gradient of efficacy of the 3 types of leaf extracts tested for antifungal efficacy against C. falcatum was: mixed leaf extracts leaf> neem leaf extracts> bitterleaf leaf extracts, regardless of the extractant at all the concentrations used in this study.
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Keywords
Antifungal, aqueous, bitterleaf, concentrations, ethanolic, extracts, extractant, mixed, neem, red rot.
Citation
Olahan et al. (2020). Bioefficacy of leaf extracts of Azadirachta indica and vernonia amygdalina and their mixture against Colletotrichum falcatum. Annals of West University of Timisoara. ser. Biology, 23(1): 83 - 88.