Biofilm Detection and Antibacterial Susceptibility Pattern of Isolates from In-patients with Urinary Tract Infection in a Tertiary Hospital
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Date
2018-12
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Journal ISSN
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Publisher
Journal of Pharmaceutical Research, Development & Practice
Abstract
A survey for the detection of biofilms amongst uropathogens was conducted in a Medical ward of a Tertiary
hospital in Ilorin, Kwara State. This was done to determine the prevalence of biofilm producing uropathogens
among patients with CA-UTI (Catheter-Associated Urinary Tract Infection) using MTP (microtitre plate)
method. Effect of media variations in the production of biofilms and the antibiogram pattern of the isolates were
also determined. Out of the 50 urine samples collected and analyzed, 27 (54.0 %) yielded growth and 28
isolates were identified. Klebsiella pneumoniae was the most prevalent (42.9 %), followed by Staphylococcus
aureus (35.7 %) and Pseudomonas aeruginosa was the least (21.4 %). A total of 59. 3 % of the isolates were
resistant to various antibiotics used and 39.3 % of the isolates were found to be multiple antibiotic resistant. Of
the 28 uropathogens isolated, 21 (75.0 %) were biofilm formers comprising of 17.9 % high biofilm formers and
57.1 % of moderate biofilm formers while 25.0 % did not form biofilm in BHIsuc. High biofilm formation was
observed in Staphylococcus aureus. There was variation in biofilm formation with different media as more
isolates (75. 0 %) produced biofilm in BHIsuc than in TSBglu (67.9 %). Clinical isolates of Staphylococcus
aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa have potential of biofilm production which could
lead to relapse of disease condition and eventually treatment failure in CA-UTI.
Description
Keywords
CA-UTI, Biofilm, Multiple Antibiotic Resistance