Naringenin upregulates the expression of CYP17 and CYP19 in the rats' testes: any effects on sperm functionality?

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dc.contributor.authorAdana, M. Y.
dc.contributor.authorAkang E. N.
dc.contributor.authorEche, S.
dc.contributor.authorUgochukwu, O.
dc.contributor.authorAzu, O. O.
dc.date.accessioned2025-05-22T14:00:22Z
dc.date.available2025-05-22T14:00:22Z
dc.date.issued2020
dc.description.abstractBackground: CYP17A1 and CYP19A1, are members of the cytochrome P450 superfamily which are monooxygenases that catalyze many reactions involved in steroidogenesis. In humans, the gene CYP17, located on chromosome 10q24.3 , encodes the 17 alpha-hydroxylase while CYP19 gene which resides on chromosome15q21.1 encodes aromatase enzyme . They are both expressed in the gonads. Optimal spermatogenesis has been shown to be dependent on the expression of these genes. They are known to be crucial for germ cell development and functionality. This research studied the expression of these genes in the rats' testes and investigated the effects of Naringenin, a bioactive flavonoid on their expression. Methods: Thirty male rats weighing 200-220g, were randomly assigned into 3 treatment groups- DW: Distilled water, N40: Naringenin, 40 mg/kg, N80: Naringenin, 80 mg/kg. Treatment lasted for a period of 70 days. Rats were then sacrificed, blood samples collected for hormonal assay, testes were harvested and semen were analysed. Expressions of CYP 17 and CYP 19 genes were done via real-time PCR. The Animal Research Ethical Committee, UKZN, South Africa approved this research (reference: AREC/046/016D). Results: The animals in groups N40 and N80 displayed significantly higher expression of CYP 17 and CYP 19 genes when compared to controls. Serum testosterone and LH levels were also significantly higher. However, no significant differences in the sperm count and percentage of abnormal sperms observed across the groups. But there were significantly lower progressive sperms in group N40 when compared to control. Conclusion: The study suggests that even though Naringenin displayed increased expression of CYP 17 and CYP19 genes in the testes, there was no significant impact on the semen parameters and no effects on the overall testicular function. Naringenin, a bioflavonoid may be able to protect against testicular toxicity but does not improve the function of an otherwise healthy testes.
dc.identifier.issn1742-8149
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/123456789/16794
dc.language.isoen
dc.publisherTaylor & Francis Group
dc.subjectantiretroviral therapy
dc.subjectsemen parameters
dc.subjectmale infertility
dc.subjectCYP17A1
dc.subjectCYP19A1
dc.titleNaringenin upregulates the expression of CYP17 and CYP19 in the rats' testes: any effects on sperm functionality?
dc.typeArticle

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