Production of Collagenases by Aspergillus terreus and Aspergillus flavus Isolates Using Scales of Tilapia and Croaker Fishes.
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Date
2020
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Journal ISSN
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Publisher
Cameroon Bioscience Society.
Abstract
Collagenase producing microorganisms are crucial for efficient utilization of collagen in food and pharmaceutical industries and fish scales play vital role as inducers. Collagen degrading fungi were isolated from fish scale waste dumps. Proximate analysis of the scales of Tilapia (Oreochromis niloticus) and Croaker (Pseudotolithus senegalensis) fishes was determined. Ground scales of the fishes were separately used in submerged fermentation for the production of collagenase by isolated fungi. Fermentation conditions were varied and the factors that favored highest yields were combined in a single process. Protein was found to be the highest component of the scales of Tilapia (62.36%) and Croaker (54.29%) fishes. Collagenase activities of 0.326 U/mL and 0.277 U/mL from Aspergillus flavus and 0.269 U/mL and 0.245 U/mL from Aspergillus terreus were produced respectively on Tilapia and Croaker scales. Activities were optimized at 37 °C, pH 7.5, and 1% substrate concentration in the presence of CaCl2 salts with Tilapia scales resulting in a significant improvement up to 31%. In conclusion, A. flavus and A. terreus have collagenolytic activity and the scales of Tilapia and Croaker fishes are suitable as substrate for collagenase production.
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Keywords
Aspergillus flavus, Aspergillus terreus, Collagenase, fish scales
Citation
Omojasola, P.F. Adeniran, F., Saliu, B.K., and Kazeem, M.O. (2020) Production of Collagenases by Aspergillus terreus and Aspergillus flavus Isolates Using Scales of Tilapia and Croaker Fishes. Cameroon Journal of Biological and Biochemical Sciences 28(2): 131-145.