Phytochemical, Antioxidant and Antimicrobial activities of the Hydroalcoholic Extract of the Roots of Adenodolichos peniculatus against Selected Pathogenic Microorganisms

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The Pharmaceutical Society of Kenya


Drugs derived from natural sources present a significant alternative therapy in the management of infectious diseases. The increasing resistance of pathogenic microorganisms to orthodox medicine has necessitated the search for alternative means of treatment. This study aimed at evaluating the phytochemicals, antioxidant and antimicrobial activities of the hydroalcoholic extract of the roots of Adenodolichos paniculatus against selected pathogenic microorganisms. Evaluation of phytochemical constituents was done using standard procedures and the antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl. Antimicrobial activity was investigated using fifteen pathogenic organisms, which included five gram-positive and seven gram-negative bacterial strains and three fungal strains using Agar diffusion method. The zones of inhibition of the extract against the test organisms, their minimum inhibitory concentrations (MIC) as well as the minimum bactericidal/fungicidal concentrations (MBC/ MFC) were determined. The extract was found to contain steroids, flavonoids, alkaloids and saponins. The total phenols, flavonoids, alkaloids and saponins were found to be 1.427±0.02 mg/g gallic acid, 0.4239±0.19 mg/g quercetin, 5% and 75.23% respectively. The antioxidant activity at concentrations of 7.53 and 15.51 µg/mL were not statistically different from that of vitamin C (P<0.05) and the IC50 (Half-maximal inhibitory concentration) found to be 4.906µg/mL. The zones of inhibition ranged from 22 â 47 mm while the MIC and MBC/MFC was observed to be 5 to 10 mg/ mL, and 10 to 40 mg/mL respectively. The availability of phytochemicals with remarkable antioxidant activities and the demonstration of antimicrobial activity against gram-positive, gram-negative bacteria and fungi strains show that A. paniculata root is a potential source for the production of antioxidants as well as antimicrobial drugs.



Adenodolichos peniculatus; Antimicrobial activity; Antioxidant activity; Phytochemiocal; Zone of inhibition; Hydroalcoholic extract;