MODELS OF DAMAGES CAUSED BY MICROSPORIDIA INFECTION AMONG HIV-POSITIVE PATIENTS IN WHITE ALBINO MICE (mus musculus domesticus)
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Date
2025-03-01
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JOURNAL OF BIOMEDICAL INVESTIGATION (JBI)
Abstract
Background: Microsporidiasis has been
reported mostly in patients with Human-
Immuno Deficiency Virus (HIV)/AIDS with
diarrhea and in more than 50% of these
patients, no enteric pathogen are identifiable.
Aim: This study attempted to establish the
natural route of human infection of
microsporidiasis and study the
pathologically induced manifestations in
experimentally infected mice.
Methods: We isolated microsporidia spores
from stool specimens of HIV-infected
patients aged 2-61years and experimentally
infect Albino mice with these isolates in an
attempt to establish the natural route of
human infection and study the
pathologically induced manifestations in
these experimentally infected mice. Seven
hundred and fifty stool samples wereand
375 samples from their non-infected
counterparts to determine the prevalence of
Microsporidia infection. Chromoptrope 2R
and Ficoll-Hipaque techniques were used to
isolate Microsporidia spores. Purification of
these isolates was conducted using sucrose
gradient centrifugation technique.
Prednisolone was used to suppress the
immune system in the experimental albino
mice. Immuno-compromised and immuno-
competent albino mice were orally,
intranasally and intravenously inoculated
with 55 spores/10μl in Phosphate Buffer
Saline. At intervals of 2, 5, 10, 17 and 28
days post-infection, the infected mice were
sacrificed and their internal organs
processed for histopathological studies,
using heamatoxylin and eosin stain.microsporidiasis with overt pathology in
vital internal organs have been demonstrated
in the immuno-suppressed mice.
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Citation
Models, Damages, Microsporidia, HIV-Positive, White Albino Mice