Isolation and Antimicrobial Activity of β-Sitosterol-3-OGlucoside from Lannea Kerstingii Engl. & K. Krause (Anacardiacea)

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Nitte University


The emergence of more and more drug resistance bacteria has led to the study of the antimicrobial activity of the compound isolated from Lannea kerstingii Engl. & K. Krause (Anacardiacea) since the active principles of many drugs found in plants are secondary metabolites.Acompoundwas isolated using dry vacuumliquid chromatography and elutingwith CHCl -EtOAc andmonitored using TLC. 3 1 13 The glycoside was characterized using H NMR and C NMR spectra recorded in DMSO-d6 at 400 MHz and 125 MHz, respectively. The antimicrobial activity of the compoundwas determined using agar diffusionmethod. Theminimuminhibitory concentration (MIC) and minimum bactericidal/minimum fungicidal concentration (MBC/MFC) was determined using broth dilution method. The compound isolatedwas found to be β-sitosterol-3-O-glucoside. The β-sitosterol-3-O-glucoside (200μg/ml)was active against S. aureus, Methicillin Resistant Staphylococcus aureus, P. mirabilis, S. typhi, K. pneumoniae, E. coli, B. subtilis with zone of inhibition ranging from 24mm to 34mmand inactive against P. aeroginosa and Proteus vulgaris. Itwas also active against the fungi C. albicans and C. tropicalis but inactive against C. krusei. The MIC ranged from25 to 50 μg/ml while the MBC/MFC ranged from50 to 200 μg/ml. These results show the wide spectrumantimicrobial activity of β-sitosterol-3-O-glucoside.



sitosterol, glycoside, Lannea kerstingii, isolation