Biodegradative activities of some gram- negative bacilli isolated from kerosene treated soil grown with cowpea (Vigna unguiculata)

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dc.contributor.authorADETITUN, DAVID
dc.contributor.authorAWOYEMI, OLUSHOLA
dc.contributor.authorADEBISI, OLUSOJI
dc.contributor.authorKOLAWOLE, OLATUNJI
dc.contributor.authorOLAYEMI, ALBERT
dc.date.accessioned2018-03-27T15:30:24Z
dc.date.available2018-03-27T15:30:24Z
dc.date.issued2016
dc.description.abstractThe biodegradative activities of three Gram negative bacilli (Aeromonas hydrophila, Vibro parahaemolyticus and Actinobacillus sp) isolated from soil contaminated with kerosene and planted with cowpea was investigated. The isolates were identified using MicrobactTM ID 24E system for the identification of Enterobacteriaceae and common miscellaneous Gram negative bacilli (MGNB). 2kg of soil collected from University of Ilorin was placed inside transparent, drilled buckets. Physicochemical properties of the soil were recorded. The soil was contaminated with kerosene at different concentrations of 0ml, 7ml, 14ml, 21ml, 56ml, 112ml, 168ml and 224ml. The setup was laid out in a randomized complete block design with three replicates. Cowpea seeds of the variety Samaru-40 were cultivated and observed for eight weeks. The results indicated that kerosene contamination of soils significantly affected the growth parameters: germination percentage, time of germination, rate of germination, plant height, number of leaves, leaf area and root nodules. A negative interaction existed between the level of the contaminant and the growth characteristics measured. Their biodegradative activities were studied and confirmed by the change in the Total Petroleum Hydrocarbon (TPH) using gravimetric method. The biodegradative abilities of the isolates were compared by measuring the optical densities, total viable count, pH and emulsification activity. The results showed that the organisms did better as a consortium rather than singly. Aeromonas hydrophila had the highest biodegradative activity followed by Vibro parahaemolyticus and then Actinobacillus sp. The study recommends the use of Aeromonas hydrophila and consortium for more effective biodegradation.en_US
dc.description.sponsorshipNilen_US
dc.identifier.citation1en_US
dc.identifier.urihttp://hdl.handle.net/123456789/190
dc.language.isoenen_US
dc.publisherAgrosearchen_US
dc.relation.ispartofseries16;1
dc.subjectBacterial Biodegradationen_US
dc.subjectSoilen_US
dc.subjectCowpeaen_US
dc.subjectKeroseneen_US
dc.subjectTotal Petroleum Hydrocarbonen_US
dc.titleBiodegradative activities of some gram- negative bacilli isolated from kerosene treated soil grown with cowpea (Vigna unguiculata)en_US
dc.typeArticleen_US

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