Cofactor interactions in the activation of tissue non-specific alkaline phosphatase: Synergistic effects of Zn2+ and Mg2+ ions
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Date
2007
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Journal ISSN
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Publisher
Nigerian Society for Experimental Biology
Abstract
The interactions of Mg2+ and Zn2+ ions in the activation of non-specific tissue alkaline
phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline
phosphatase by the metal ions was accompanied by changes in the kinetic parameters of p
nitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ions in promoting the hydrolysis of p-nitrophenylphosphate by alkaline
phosphatase. The results show that assays of alkaline phosphatase activity in homogenised tissue samples will give better responses if both Mg2+ and Zn2+ ions are included in the reactions.
Description
Keywords
Alkaline phosphatase, kinetics, Enzyme-cofactor interaction, synergism