Ameliorative Effect of Aqueous Leaf Extract of Cola Acuminata on Mercury Chloride-induced Neurotoxicity in Male Wistar Rat
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Date
2024
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Faculty of Pharmacy, Cairo University, Egypt
Abstract
Aim: The ameliorative effect of aqueous leaf extracts of Cola acuminata (ALECA) on mercury chloride (HgCl2)-induced neurotoxicity in male Wistar rats was investigated. Methods: Thirty-six male Wistar rats weighing 120e150 g were randomly divided into six groups; group I received 0.5 ml normal saline; group II received 5 mg/kg body weight HgCl2; group III received 5 mg/kg HgCl2 150 mg/kg body weight ALECA;group IVreceived 5 mg/kg HgCl2 300mg/kg ALECA;groupVreceived 5mg/kg HgCl250 mg/kgN-acetyl cysteine (NAC); and group VI: received 5 mg/kg HgCl2 500 mg/kg body weight vitamin C. The animals were treated for 21 days through the oral route. The rats were subjected to open field tests and forelimb grip tests on days 14 and 21. The animals were then killed and brain tissues were collected, homogenized, and centrifuged for measurement of biochemical parameters: tumor necrosis factor alpha and nitric oxide. Results: The data obtained from the forelimb grip test showed that muscular strength was higher in the group given ALECA,NAC,andvitamin Cwhencomparedwiththegroupgiven onlyHgCl2 (P˂0.05). The data from the open field test also showed that locomotory activity and exploratory behavior are higher while anxiety levels are lower in the group treated with ALECA, NAC, and vitamin C when compared with the HgCl2 group (P˂0.05). Also, the levels of tumor necrosis factor alpha and nitric oxide are lower in the group treated with ALECA when compared with the HgCl2 group (P˂0.05). From the results, it was established that ALECA-treated groups compared favorably with the two reference drugs. Conclusions: These findings show the ameliorative effect of ALECA on mercury chloride induced neurotoxicity/ brain damage through modulation of inflammatory pathway. Keyw
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Keywords
Cola acuminate, Mercury chloride, Neurotoxicity, Nitric oxide, Tumor necrosis factor
Citation
Ayinla M.T., Yakub Z. O., Asuku A.O and Muzzamil S.S. (2024). Ameliorative Effect of Aqueous Leaf Extract of Cola Acuminata on Mercury Chloride-induced Neurotoxicity in Male Wistar Rat. Bulletin of Faculty of Pharmacy Cairo University, Vol. 62 (1), Article 2. 11-18. Available online at: https://www.bfopcu.eg.net/journal/vol62/iss1/ https://www.bfopcu.eg.net/journal/vol62/iss1/2