THE ROLE OF ASCORBIC ACID AS AN ANTIOXIDANT AGAINST GENOTOXICITY OF ZINC OXIDE NANO-PARTICLES ON TISSUES OF HETEROBRANCHUS LONGIFILIS
No Thumbnail Available
Date
2025-04-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The indiscriminate discharge of effluents from Zinc oxide nano-particles (ZnO-NPs)
industries into water bodies can cause genetic disorders in fish. The genotoxicity of ZnO
NPs was investigated on Juveniles of Heterobranchus longifilis through exposure to sub
lethal concentrations (6.00, 8.00, 10.00, 12.00 mg/L) of ZnO-NPs for 60 days, and fish
were fed on ascorbic acid (50.00, 250.00, 500.00, 1000.00 mg AA/kg diet) for 30 days.
After each experiment, the micronuclei (MN) abnormalities and deoxyribonucleic acid
(DNA) quality were evaluated. The commonest abnormalities recorded are micronucleated,
binucleated, multinucleated, altered, echinocyte cells, kidney-shaped and deformed nuclei.
However, groups ameliorated with a 500 mg AA/kg diet recorded little or no abnormalities.
A dose and time-dependent increase in MN induction and frequency occurred compared to
control but decreased (p<0.05) as the concentration of AA increased. There were highest
MN induction and frequencies in the liver (38.23%) than in gills (26.32%) and blood
(24.61%) of ZnO-NPs-exposed fish, with the lowest MN induction and frequency in the
liver (10.21%), gills (1.76%) and blood (1.61%) of 500 mg AA/kg diet-group. DNA quality
reduced with increasing concentrations and exposure periods but improved in the 500 mg
AA/kg diet group. This indicated that ZnO-NPs are toxic, but 500 mg AA/kg-diet was able
to minimize MN induction and DNA damage in H. longifilis.
Keywords: Amelioration, Ascorbic acid, DNA, Micronucleus, Zinc oxide nano-particles, Heterobranchus longifilis
longifilis longifilis