Kolaviron protects the prefrontal cortex and hippocampus against histomorphological and neurobehavioral changes in cuprizone model of multiple sclerosis.
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Malaysian Journal of Medical Sciences
Abstract
Background and Aim: This study explored the efficacy of kolaviron (kv)-a biflavonoid complex isolated from the seeds of Garcinia kola in protecting against Cuprizone (CPZ)-induced demyelination in both prefrontal cortex and hippocampus of Wistar rats.
Methodology: Thirty rats were treated to receive (A) 0.5 ml PBS (Control); (B) 0.5 ml corn oil; (C) 0.2% CPZ; (D) 0.2% CPZ and 200 mg/kg of kv and (E) a combination of 200 mg/kg of kv and 0.2% CPZ for 6 weeks each. Rats were assessed for exploratory functions and anxiety-like behaviour before being euthanised and perfused transcardially with 4% paraformaldehyde. Prefrontal and hippocampal thin sections were stained in H&E and cresyl fast violet stains.
Results : CPZ-induced demyelination resulted in behavioural impairment as seen by reduced exploratory activities, rearing behaviour, stretch attend posture, center square entry and anxiogenic characteristics. Furthermore, degenerative changes including pyknosis, karyorrhexis, neuronal hypertrophy and reduced Nissl integrity were seen in response to CPZ administration compared to control. However, rats treated with kv before or after CPZ administration showed significant improvement in behavioural outcomes and comparatively normal cytoarchitectural profile in neural tissues.
Conclusion: This study showed that kv provides protective roles against CPZ-induced neurotoxicity through prevention of ribosomal protein degradation.
Description
Keywords
cuprizone,, Kolaviron, demyelination, neurohistomorphology, Neurobehaviour
Citation
25(2):50–63.