Hepatoprotective potential of Phyllanthus muellarianus leaf extract: studies on hepatic, oxidative stress and inflammatory biomarkers,
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Informa UK limited, trading as Taylor & Francis group
Abstract
ABSTRACT
Context: Leaves of Phyllanthus muellarianus (Kuntze) Exell. (Euphorbiacea) are widely used in the management of liver disorders in Nigeria. However, no there is no scientific validation to support this use. Objective: Hepatoprotective effect of Phyllanthus muellarianus aqueous leaf extract was investigated in acetaminophen-induced liver injury mice.
Materials and methods: Hepatoprotective effect of Phyllanthus muellarianus aqueous leaf extract was evaluated in acetaminophen-induced hepatic damage in Swiss albino mice using biomarkers of hepatocellular indices, oxidative stress, proinflammatory factors and lipid peroxidation. Mice received distilled water, 100, 200, or 400 mg/kg b.w of Phyllanthus muellarianus aqueous leaf extract, respectively, for seven days.
Treatment groups were challenged with 300 mg/kg b.w of acetaminophen on the sixth day.
Results: Oral administration of Phyllanthus muellarianus aqueous leaf extract significantly (p < 0.05) attenuates acetaminophen-mediated alterations in serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin and total bilirubin by 76.56, 85.41, 89.39, 82.77 and 78.38%. Similarly, acetaminophen-mediated decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase were significantly attenuated in the liver of mice by 85.10, 80.81, 80.45, 76.23 and 95.22%, respectively. Increased levels of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl, fragmented DNA, tumor necrosis factor-a, interleukin6 and -8 were significantly lowered by Phyllanthus muellarianus aqueous leaf extract.
Conclusion: Overall, results of this study show that Phyllanthus muellarianus halted acetaminophenmediated hepatotoxicity due to its capability to enhance antioxidant enzymes.
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Keywords
Acetaminophen; antioxidant enzymes; fragmented DNA; lipid peroxidation; protein oxidation; proinflammatory biomarkers
Citation
https://www.tandfonline.com/toc/iphb20/55/1